Background: Visfatin, also known as nicotinamide phosphoribosyltransferase, has been suggested as a pro‑ inflammatory and immunomodulating marker for PERIODONTITIS. The aim of this study was an immunohistochemical analysis of visfatin in gingival tissues of patients with chronic PERIODONTITIS and aggressive PERIODONTITIS. Materials and Methods: In this cross‑ sectional study based on clinical evaluation and inclusion and exclusion criteria. Twenty patients with generalized chronic PERIODONTITIS, 13 patients with generalized aggressive PERIODONTITIS, and 20 periodontally healthy individuals enrolled. Gingival tissue samples were obtained during periodontal flap surgery and crown lengthening surgery in periodontal patients and healthy group, respectively. Tissue samples were transferred to a pathology laboratory to determine the degree of inflammatory infiltration by hematoxylin and eosin staining and the level of visfatin expression by immunohistochemistry. The data were analyzed using SPSS version 20 statistical software and paired t‑ test, Mann– Whitney test, and Spearman rank correlation coefficient. P < 0. 05 was considered statistically significant. Results: Inflammation grading and visfatin expression were significantly higher in periodontally diseased gingiva compared to the control group (P < 0. 05). However, no significant difference was found between two diseased groups. The relationship between inflammation grading and visfatin expression in aggressive PERIODONTITIS group was statistically significant, positive and relatively strong (P = 0. 025, r = 0. 617). However, no significant relationship has been found between visfatin expression and inflammation grading in the chronic periodontitits and control groups (P > 0. 05). Conclusion: Visfatin expression was increased in gingival tissues of chronic PERIODONTITIS and aggressive PERIODONTITIS patients. Hence, visfatin may have a role in the etiopathogenesis of chronic PERIODONTITIS and aggressive PERIODONTITIS.