Pulses form stable dietary components for majority of population across the world. Different people consume pulses in different ways like Uncooked, Soaked, and cooked or only cooked without soaking. All these processing techniques lead to changes in the NUTRITIONal value of the pulses. Studies have also reported that in addition to NUTRITIONal components like Proteins, Carbohydrates, and fats, pulses also contain anti-NUTRITIONal components like Lectins, Tannins, and Polyphenols that greatly interfere with digestion of pulses in the human intestine. Hence in the current study a comprehensive review is being compiled to evaluate the NUTRITIONal and antiNUTRITIONal aspects of pulses and effect of processing methods on invitro protein and starch digestibility of the pulses.

This study aimed to investigate the effect of different culture mediums based on the Walne medium on the growth rate, chemical composition, and fatty acid (FA) profile in I. galbana and N. oculata. This experiment was done in a factorial design with two culture media (Walne and modified Walne media) and two microalgae species, including I. galbana and N. oculata. The results showed that the modified culture medium increased total and daily fresh and dried biomass production of I. galbana and N. oculata. Modified culture media increased crude fat (CF) and crude protein (CP) content and total and daily lipid production in both the studied species. However, in both the studied culture mediums, N. oculata had higher growth and production performance compared to I. galbana. Modified growth media also affects the FA profiles of the studied microalgae species. Total saturated and unsaturated FA content was not influenced by the growth medium but modified media increased poly unsaturated FA (PUFA) at the expense of mono unsaturated FA (MUFA). Omega- 3 FA content (linolenic acid, Eicosapentaenoic acid (EPA), Docosahexaenoic acid (DHA)) was increased as a result of the medium modification in both species. However, linoleic acid content was affected differently in I. galbana and N. oculata. The linoleic acid concentration was reduced in modified medium grown N. oculata but increased in I. galbana. Palmitic acid and stearic acid contents were also decreased in both of the studied species in the modified medium. This study develops microalgal cultivation using a modified Walne medium for higher CP, CF, EPA, DHA contents, the ratio of omega3: omega6 FA, and biomass production in N. oculata and I. galbana microalgae.Microalgae species can produce oil and protein in non-cultivable lands, reducing the need for defrosting and can have an important role in reducing carbon foot-print of animal production. One of the most valuable products of microalgae is their oil, which ranges from 20 to 50% of the dry weight of microalgae (Brennan and Owende 2010; Leonga et al. 2018). The I. galbana is often grown on farms to produce oils that contain large amounts of PUFA rich in omega-3 long chain FA, such as EPA and DHA (Gouveia et al. 2008). Species of the genus N. oculata are also known to be rich in EPA (Kagan et al. 2014; Borges et al. 2016). Factors such as nutrient quantity and quality, light, pH, turbulence, salinity, and temperature are the most important parameters on which, the growth of microalgae depends (Lavens and Sorgeloos 1996; Converti et al. 2009; Emmanuel and Nelson 2016). Vitamins regulate biochemical reactions in microalgae (Hakalin et al. 2014) and the growth rate of some microalgae species is highly dependent on some the vitamins such as cobalamin, biotin and thiamine (Tandon et al. 2017). However, the effect of vitamins on the growth, diversity, and productivity of microalgae has been poorly studied (Arif et al. 2019). The effects of different Nitrogen and phosphorus concentration as the main limiting nutrients on growth performance and biomass production of I. galbana and N. oculata had been evaluated previously (Andersen 2005; Zarrinmehr et al. 2020). However, there was not any report about effects of modifying the availability of culture medium sources of nitrogen and phosphorus. In the composition of the Walne medium, No3-, Po4-, and Cl- are considered as anions, and Na+, Mn+, Co+, Zn+, and Cu+ are considered as cations. Cations chelating anions and making them less available to microalgae. So, we hypothesize that changing the concentration of cations in the culture media, without increasing the concentration of N and P sources, will change the availability of anionic compounds for microalgae and consequently affects microalgal production and composition. Therefore, the effects of lower levels of cations including ZnCl2, CoCl2. 6H2O, (NH4)6Mn7O24, and CuSo4. 5H2O and higher levels of B1 and B12 vitamin in Walne medium on the growth rate, biomass production performance, chemical composition, and fatty acid profile of I. galbana and N. oculata, were investigated in this study. This study aimed to investigate the effect of different culture mediums based on the Walne medium on the growth rate, chemical composition, and fatty acid (FA) profile in I. galbana and N. oculata. This experiment was done in a factorial design with two culture media (Walne and modified Walne media) and two microalgae species, including I. galbana and N. oculata. The results showed that the modified culture medium increased total and daily fresh and dried biomass production of I. galbana and N. oculata. Modified culture media increased crude fat (CF) and crude protein (CP) content and total and daily lipid production in both the studied species. However, in both the studied culture mediums, N. oculata had higher growth and production performance compared to I. galbana. Modified growth media also affects the FA profiles of the studied microalgae species. Total saturated and unsaturated FA content was not influenced by the growth medium but modified media increased poly unsaturated FA (PUFA) at the expense of mono unsaturated FA (MUFA). Omega- 3 FA content (linolenic acid, Eicosapentaenoic acid (EPA), Docosahexaenoic acid (DHA)) was increased as a result of the medium modification in both species. However, linoleic acid content was affected differently in I. galbana and N. oculata. The linoleic acid concentration was reduced in modified medium grown N. oculata but increased in I. galbana. Palmitic acid and stearic acid contents were also decreased in both of the studied species in the modified medium. This study develops microalgal cultivation using a modified Walne medium for higher CP, CF, EPA, DHA contents, the ratio of omega3: omega6 FA, and biomass production in N. oculata and I. galbana microalgae.

This experiment was designed to survey the nutritive value and estimate protected-protein level of Protanomix as a new protein source and compare it with soybean meal. Protanomix is a brown powder by separating the precipitate produced from the reaction of tannin with corn-steep liquor. Crude protein (CP) and metabolizable energy (ME) in this protein source is nearly similar to soybean meal, and more than 80% of its protein content is insoluble. In this study, potential of in vitro gas production in Protanomix was significantly (P˂0.001) lower than the control treatment (soybean meal); however, substitution of soybean meal at different levels of Protanomix in experimental diets did not lead to significant changes in gas production parameters (P˃0.05). Comparing Protanomix with soybean meal (control) showed that in vitro truly degraded dry matter (IVTDDM), estimated metabilizable energy (ME) and pH were similar (P˃0.05). However, short chain fatty acids (SCFA), apparently in vitro organic matter digestibility (IVOMAD) and ammonia nitrogen (N-NH3) were significantly higher in soybean meal (P˂0.01) than Protanomix, while partitioning factor (PF), microbial protein synthesis (MPS) and efficiency of microbial protein synthesis (EMPS) were greater in Protanomix than soybean meal (P˂0.05). Nonetheless, when soybean meal was substituted with different levels of Protanomix in the experimental diets, only concentration of ammonia nitrogen (N-NH3) was significantly decreased (P˂0.001).

What is Early Life NUTRITION (ELN)? It is the NUTRITION from pre-conception to 3-5 years. The first 1000 days refers to the period from the moment of conception to when a child is aged 24 months. Why the first 1000 days and ELN are so important? Providing the right NUTRITION is essential to ensure optimum development of infants. There is also growing scientific evidence that shows that early life NUTRITION also influences susceptibility to obesity and other non-communicable diseases (NCD's) such as diabetes later in life. What is Early-life NUTRITIONal Programming (ENP)? The concept that providing the right quantity and quality of nutrients at the right time supports optimal development/ programming of the body's system and life-long health. This can be achieved through physiological (metabolism, brain and immune system) and behavioral (feeding habits, taste preferences) programming. The health of mothers before and during pregnancy, and NUTRITION and growth in fetal and early post-natal life, determine the structure, function and adaptive capacities of key organ systems. As a consequence, NUTRITIONal imbalances early in life can have long lasting programming effects on later health and risk of disease. The environment has much more impact on your health in later life than your genes. It has been estimated that at most 20% of lifelong health can be explained by inherited genes. This means that at least 80% of disease risk in later life is due to the environment, including NUTRITION and life style. How does it work? There are a number of mechanisms by which this might occur. There is growing evidence from the field of epigenetics that environmental factors, including NUTRITION, can trigger certain gene expressions which can influence our risk of developing non-communicable diseases such as diabetes and obesity later in life.The first 1000 days offer a unique window of opportunity to build long-term health. The right NUTRITION during this critical period really matters.