In this paper, the elastic modulus of NANO cellulose/PLA NANO COMPOSITES obtained by the two methods including NANO indentation and tensile tests were analyzed. NANO cellulose was extracted by Mechanical method from linter pulp fiber. Amount of usage of NANO cellulose was 3 and 5% wt, and master batch technique was used for improving NANOparticles distribution in polymer matrix. Then the mechanical properties of NANO COMPOSITES with and without this technique were studied. Tensile test was performed in accordance with the standard method, and the atomic force microscope in peak force tapping mode was used for NANO indentation. Tensile test results showed that the use of master batch improves tensile modulus, tensile strength and strain at break. Also, by increasing NANO cellulose percentage from 3 to 5% in NANO composite with master batch, the tensile strength and strain at break increased. But this increase had no significant effect on tensile properties of NANO composite without master batch. A similar trend of strength test results was observed in NANO indentation results. Based on this result, use of master batch in NANO composite caused the increase in elastic modulus. The results of these two analyses were compared and tensile test showed lower modulus value than NANO indentation.