Background: Aloe vera is a medicinal herb used as an anti-inflammatory and sedative agent.Objectives: The current study aimed to evaluate the effect of Aloe vera aqueous extract on MORPHINE withdrawal symptoms in MORPHINE-dependent female rats.Patients and Methods: The current research was performed on 40 female Wista-Albino rats which were made dependent on MORPHINE using Houshyar protocol and were randomly divided into five groups (A, B, C, D, and E). Group A did not receive any agent in the period of handling but other groups (B, C, D and E) received 5, 10, 20 and 40 mg/kg of Aloe vera aqueous extract by gavage, three times daily for a week, respectively. Withdrawal symptoms, stool form, agitation, disparity, floppy eyelids, and body mass variations were checked for 10 days. The obtained data were analyzed using SPSS v.11 software, and Friedman, Kruskal-Wallis, and Mann-Whitney statistical tests. Statistical difference was considered significant (P<0.05).Results: The results of the present study showed that agitation, disparity, and floppy eyelids in group E were significantly higher than those of others groups, however, these symptoms in group C were significantly lower than those of the other groups.Conclusions: The results of the present study revealed that the Aloe vera aqueous extract had various effects on MORPHINE withdrawal syndrome in MORPHINE-dependent female rats.