Gold nanoparticles are promising materials for biomedical applications because of the attractive optical properties such as the absorption and scattering of light at resonant wavelength. Due to the fruitful applications of gold nanoparticles (GNPs), they are appropriate for a variety of biological studies. One of the most important applications of nanoparticles is protein carriers, in which transport of therapeutically relevant protein is done to in vivo or in vitro targets. Protein folding and properties of probable intermediates during the folding of proteins had been investigated in several studies. The MOLTEN GLOBULE state, a main intermediate of protein folding, has native-like secondary but perturbation of tertiary structure. Consequently, the influence of gold nanoparticles concentration on a model protein was studied by far-and near-UV circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), intrinsic fluorescence emission spectroscopy and 8-Anilino-1naphthalenesulfonic acid binding. The results indicated that the interactions between gold nanoparticles and lysozyme lead to the formation of MOLTEN GLOBULE-like state.