MOLECULARly IMPRINTED POLYMERS are cross linked POLYMERS have selectivity around shape, structure, kind and number of templates molecules. These POLYMERS show stability in different range of pH, temperature and organic solvents. As These POLYMERS application we can mention to their usage as steady solid phase in liquid chromatography, electro chromatography, solid phase extraction and micro solid phase extraction.

Metallothioneins (MTs) are a super-family of low MOLECULAR weight cysteine-rich proteins. Although many research works have been reported on their structure, application, etc., there is not any article reported on IMPRINTED mechanism of MT-IMPRINTED POLYMERS, yet. These POLYMERS are prepared by using acrylic acid (AA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, 2,2¢-azobis(2-methylpropionitrile) (AIBN) as an initiator, and chloroform as a solvent. The IMPRINTED mechanism is first studied by infrared spectroscopy analysis, Scatchard analysis, and TEM technique. Infrared spectrograms indicate the functional groups of SH in thiolate sulphurs have lone pair electrons which can form P-P bond with C=O in AA. Scatchard plot shows at least three kinds of binding sites exist in MTIMPRINTED polymer and TEM technique indicates that most terminal thiolate sulphurs in MT are polymerized mostly as binding sites during the polymerization. The study will offer experimental foundation for further research and application of MT-IMPRINTED POLYMERS.

Background & Objectives: Biosorbents do not show desirable performance under undesirable conditions. MOLECULARly IMPRINTED POLYMERS (MIPs) have thus been proposed to resolve this issue. In an attempt for specific absorption of trace triazine herbicides, an MIP was synthesized, optimized, and used as a novel tool for MOLECULARly IMPRINTED solid phase extraction (MISPE).Methods: Atrazine and ametryn POLYMERS were synthesized using the central composite design. These POLYMERS, along with control samples, were evaluated in terms of the amount of functional monomer, template molecule, cross-linker, initiator, solvent, and polymerization temperature. The appropriate cartridge was then selected and the response surface method was applied to optimize the MISPE based on the amount of absorbent and the volume, concentration, flow rate, and pH of the sample. Finally, the concentration factor and reusability of the cartridge were examined.Results: The optimized temperature for ametryn and atrazine was calculated as 40.86oC. The solvent, cross-linker, initiator, monomer, and template molecule were 6.41 and 5.03 ml, 27.070 and 21.320, 2.03 and 2.27 mmol, 5.41 and 4.73 mmol, and 1.204 and 0.811 mmol, respectively. The results of MISPE optimization of ametryn and atrazine suggested a recovery rate of over 90% for drinking water and urine.Conclusion: The results showed that the central composite design can be used as a general tool for polymer synthesis and optimization and MISPE protocol. Furthermore, due to their high performance and selectivity, POLYMERS can assume an important role in workplace monitoring.

In this research for the first time, an adsorbent based on MOLECULARly IMPRINTED POLYMERS (MIPs) for selective extraction of Naringenin using methacrylic acid (functional polymer), trimethylolpropane trimethacrylate (cross linker), azobisisobutyronitrile (initiator) via precipitation polymerization technique in a ratio (1: 4: 20) were synthesized. After template removal, nanoporous IMPRINTED polymeric lattice was prepared which is able to extract Naringenin as a bioactive compound from the mixtures containing this component. Non-IMPRINTED POLYMERS (NIPs) with the same materials (instead of the template molecule) were synthesized to compare the efficiency of the MIPs. Selectivity of the MIPs for Naringenin has been evaluated. Quercetin as a similar molecule of the template was selected which the binding capacity for Naringenin and Quercetin were 284 mg. g-1 and 70 mg. g-1, respectively. Imaging scanning electron microscope (SEM) was used for morphological studies on the synthesized polymer particles and brannaueremmet-teller (BET) analysis method was applied to measure the porosity, specific surface area and poor diameters of the polymer.

The aim of this work was to prepare lactose IMPRINTED polymer and study of its selectivity for the recognition of different mono- and disaccharides. A series of MOLECULARly IMPRINTED POLYMERS (MIPs) against lactose were synthesized and their binding properties were compared with a Blank non-IMPRINTED polymer. Methacrylamide (MAAM) and ethylene glycol dimethacrylate were used as functional monomer and cross-linker, respectively. Dimethylsulfoxide was also applied as polymerization solvent. Different lactose: MAAM ratios were applied and optimized MIP was selected in a conventional batch adsorption study. The dissociation constant and maximum binding sites of polymer were determined using the Scatchard analysis. The selectivity of MIP for different mono- and disaccharides was also evaluated. The results indicated that the shape of cavity and orientation of functional monomers in binding sites and the spatial arrangement of hydroxyl groups in saccharide structure were responsible for the selectivity of lactose IMPRINTED polymer.

In this research a new type of MAGNETIC Pb(II) and Cr(VI) ion-IMPRINTED POLYMERS were synthesized using Fe3O4@SiO2 MAGNETIC particles as supporter, benzoyl phenyl hydroxylamine and dithizone as ligands for Pb and Cr ions respectively, 4-vinyl pyridine as functional monomer, ethylene glycol dimethacrylate as crosslinker and 2, 2′-azobis(isobutyronitrile) as the initiator. The ionic templates were achieved using HCl 2M as an eluent solution. The chemical structure, morphology, thermal behavior and MAGNETIC properties of the sorbent POLYMERS were evaluated using Fourier-transform infrared spectroscopy, scanning electron microscopy, thermal gravimetric analysis and vibrating sample magnetometry methods. Optimization of affecting parameters of the adsorption of analytes (adsorption time, pH, kind and concentration of the eluent solution, elution time and temperature) were performed using flame atomic absorption spectroscopy method. The results showed that the maximum adsorption capacities for lead and chromium at 40 ◦ C were 92. 3 and 96. 8 milligrams per gram at pH of 6 and 3 respectively. The synthesized sorbents had high selectivity and were successfully applied for these pollutants removing from waste waters in vdifferent stages of ammonium perchlorate production process.

Background: Cattle can be considered as an important source for herbicides through nutrition. Therefore, herbicide residue in animal products is a potential human exposure to herbicides causing public health problems in human life. Triazines are a group of herbicides primarily used to control broadleaf weeds in corn and other feed ingredients and are considered as possible human carcinogens. To evaluate trace residue of these pollutants MOLECULAR IMPRINTED solid phase extraction (MISPE) method has been developed, using biological samples.Methods: Blood samples were taken from the jugular vein of 45 Holstein cows in 3 commercial dairy farms in Khuzestan Province, Iran. Urine samples were also taken from the cows.Results: The mean ± SD concentrations of atrazine in serum and urine samples of the study group (0.739±0.567 ppm and 1.389±0.633 ppm, respectively) were higher (P<0.05) than the concentrations in serum and urine samples of the control group (0.002±0.005 ppm and 0.012±0.026 ppm, respectively).Conclusion: Atrazine in the feed ingredients ingested by cattle could be transferred into the biological samples and consequently can be considered as a potential hazard for the public health.