Background and Aim: Salivary gland tumors classification continuously undergoes alternations, especially in the recent years. This study used immunohistochemical markers for distinguishing between polymorphous LOW-grade adenocarcinoma (PLGA) and adenocarcinoma carcinoma (AdCC) which have several overlapping histological and clinical features.Materials & Methods: Ten AdCC and eight PLGA with five normal minor salivary glands were selected after review by two oral pathologists. LSAB method was used for seven markers: carcinoembryonic antigen, epithelial membrane antigen, muscle specific actin, vimentin, S100, P53 and Ki-67. Finally, the data were analyzed by Mann-Whiteny test.Results: Analysis of data showed a high expression of CEA, EMA and Ki-67 in AdCC compared to PLGA. Although, CEA (P Less than 0.001) expression was limited to luminal cells .In contrast, MSA (P=0.034) expression in nonluminal cells, and Ki-67 (P Less than0.001) occurred in both luminal and nonluminal cells. Also there was highly expression of vimentin and S-100 in PLGA with no significancy for EMA and Tp53. Conclusion: The result of this study suggests that ICH can be a helpful tool. The immunostaining of AdCC and PLGA with CEA, Vimentin and Ki-67 were of greater significant, S100 and MSA were less significant and the others (EMA and P53) could not offer an adjunctive aid in differential diagnosis between the two tumors.