What is Plasmid Prep?ISOLATION of plasmid DNA from E. coli is a common routine in research laboratories to purify a specific sequence, since they can easily be purified away from the rest of the genome. You will perform a widely‐practiced procedure that involves alkaline lysis of cells. This protocol often referred to as a plasmid "mini‐prep," yields fairly clean DNA quickly and easily.

Purpose: The aim of this study was to identify the causes of social ISOLATION in high school students in Gorgan city. Methods: The research method was qualitative and sampling used in this analysis was purposive sampling. The statistical population of the present study included the participating team, scientific and practical experts who had at least ten years of experience in training, consulting and policy in the field of educational management. Based on this, 10 people were selected as the final experts and the interview continued until the theoretical saturation was reached. In this study, interviews were used to collect research data. Then, using qualitative analysis of grounded theory, a number of concepts of extraction and coding of indicators were performed. Data analysis was performed using MAXQDA software. Findings: The results showed that the 6 paradigms derived from grounded theory are: indicators of effective role of parents and teachers, students' lack of language, household economic status and family personality type as contextual categories in presenting the model of social ISOLATION of high school students in Gorgan Were. Conclusion: Based on the results of qualitative data analysis of the foundation, the scope and nature of social ISOLATION in high school students in Gorgan is affected by the following categories: socioeconomic anomalies, individual variables, parental role, strengthening social relationships, psychological interactions and Family level.

What is it used for?Small RNA extraction is the first step prior to proceeding for microRNA RT-PCR. Before performing small RNA ISOLATION from total RNA (extracted in previous section), two more steps are required: 1. DNase treatment and 2. Agarose gel electrophoresis, which are respectively crucial for eliminating of DNA contamination and to check the extracted RNA quality.

Mass ISOLATION is a method of structural vibration control against environmental loads such as strong earthquakes. Buildings with local ISOLATION systems are a practical method of mass ISOLATION. These buildings can be non-proportionally damped systems. Available methods for dynamic analysis of these buildings are complex and time-consuming. In this paper, the concept and efficiency of these buildings at seismic response reduction is examined. A simple method for their dynamic analysis is introduced. Proof of the accuracy of the method is also presented.