Background: HYPERGLYCEMIA is one of the important featurs of diabetes. In cell culture studies different methods are used to mimic the HYPERGLYCEMIA condition. In this study we investigate response of human liver cancer cell line (HepG2) to high insulin, high glucose, and high insulin/ high glucose medium exposure. Methods: HepG2 cells were settled in DMEM+0. 1% FBS or DMEM free-serum medium with high concentrations of d-glucose (30 mm) and/or insulin (1μ M) for 24h after an overnight starving in serum-free medium. The level of HYPERGLYCEMIA was estimated in the supernatants via GOD-POD method. Results: Serum-free madium with high insulin/ high glucose consentration made the higher level of hypreglycemia in HepG2 cells. Conclusions: Our study interduced high insulin/ high glucose treatment as the best way to induction HYPERGLYCEMIA.

INTRODUCTION: Stress HYPERGLYCEMIA is defined as a transient increase in blood glucose concentration during an acute physiologic stress. The study was done to demonstrate the relative frequency of stress HYPERGLYCEMIA in children admitted to AL-Zahra hospital emergency department from March 1998 to December 1998.MATERIAL & METHODS: This study was cross-sectional descriptive and included 291 patients (6 months to 5 years old), all required venipuncture, who were selected by simple sampling method. Blood glucose ≥ 200 mg/dl was considered as HYPERGLYCEMIA and any clinical, diagnostic and surgical procedure that led to hospitalization of the baby was defined as stress . RESULTS: The relative frequency of HYPERGLYCEMIA, normoglycemia and hypoglycemia were 25.42%, 72.18% and 2.40% respectively. There was not any significant difference between boys and girls (P>0.05) in this regard. 130 subjects were encountered with one kind of stress and the relative frequency of HYPERGLYCEMIA was different base on the kind of stress (50% in hematologic diseases, 40% in respiratory diseases and 29.4% in gastrointestinal diseases). Relative frequency of stress HYPERGLYCEMIA among patients with 2 or more stresses was 24.22% which was not significantly different from those with one stress (P>0.05). CONCLUSION: It seems that HYPERGLYCEMIA stress is not related to the number of stresses of patients but is associated with the type of the disease.Relative frequency of HYPERGLYCEMIA stress in this study (25.42%) was differ from those reported by others (51.9% in ICU inpatients and 0.46% to 9.4% in other departments) which may be attributed to the addmission of intensive care service needed patients with other patients in emergency department in AL-Zahra hospital.

HYPERGLYCEMIA, in trauma patient, is commonly associated with a hyper metabolic stress response. Our objective is to determine the effects of HYPERGLYCEMIA on the overall outcome of head trauma patients. In this descriptive study data were collected from head trauma patients' admitted to Intensive Care Unit (ICU) of Poursina University Hospital in a one-year period (Jan 2004-Jan 2005), retrospectively. All patients had stayed in the ICU for more than 48 hours post-injuries. They were divided into two groups according to their serum glucose levels at the time of admission (<200mg/dl or >200mg/dl), age, gender and Injury Severity Score (ISS). Patients with diabetes mellitus were excluded .We determined the outcome according to duration of hospitalization and ICU stay as well as mortality rates. Variables were analyzed with t-test and chi square test. Out of 115 patients, 89.6% were men. About 36 % of patients had serum glucose levels D 200 mg/dl over the study period and this group had significantly greater mortality rate but without necessarily longer ICU or hospital stay. In this study we have shown that admission HYPERGLYCEMIA has significant t effect on patient’s mortality but it is still unclear whether it can be a cause for longer ICU/hospital stay.