Background and Aim: It is well - known that sex hormones regulate bone metabolism. Sex steroids increase osteoblasts activities and affect Growth, remodeling and bone homeostasis. The aim of this study was to examine the effects of sex hormones deficiency on craniofacial Growth in rats.Methods & Materials: Fifty, thirty-day-old Wistar rats comprised the sample in this experimental study. 25 male rats were divided into 2 groups: Experimental group, ORX, (n=15) and control group, sham- operation, (n=10) and 25 female rats were divided in the same way: Experimental group, OVX, (n=15) and control group, sham- operation, (n=10). Body length and weight were registered monthly. The rats were sacrificed 6 months after the surgery. Direct millimetric measurements of the skeletodental variables and the tibial length were obtained by using electronic caliper. Serum testosterone, progesterone and estradiol levels were measured by ELISA. One Way ANOVA, Tukey and Student t tests were used to analyze the data.Results: Serum testosterone level significantly decreased in the ORX group as compared with the male sham operated group. In the ORX group, body length and weight, coronoid height, mandibular length, mandibular arch length, midfacial width, midfacial length, midfacial height, calvarial width, maxillary arch width, premaxillary length, nasal bone height, facial width, basisphenoid bone length and tibia bone length were significantly smaller than in the male control group. Structures showing cartilaginous Growth were influenced more than structures showing sutural Growth. Estradiol level did not change in OVX group, but despite the significant decrease in progesterone level, no significant differences except weight were found between the OVX group and female control group.Conclusion: In conclusion, it is strongly suggested that the suppression of sex hormones secretion in the Growth phase might inhibit craniofacial Growth and results in poor craniofacial development and developing malocclusion.