This study was conducted to investigate abundance and species distributn of macrobenthos in 3 stations in Gorganroad River (S1: 36 58 42E, 54 00 28; S2: 36 58 41, 54 01 27; S3: 36 59 14, 54 02 54) from March to February 2013. Samples collected using Van ven grab in 3 replicates in each station. In the study area, 6 orders belonging to 9 families were indentified including Naididae, Gammaridae, Cardidae, Nereidae, Neritidae, Ampharetidae, Pyrgulidae, Ostracoda, Foraminifera, Balanidae and Cumaceae. The most abundant taxa were Ampharetidae (28. 62%), Lumbriculidae (24. 24%), Naididae (11. 3%), Chironomidae (10. 44%), Balanidae (10. 39%), Gammaridae (6. 14%), Nereididae (31. 91%), Cardiidae (2. 73%) and Tubificidae (2. 19%). Generally, in all 3 stations the highest and lowest biomass belonged to the Ampharetidae family (25410) and Tubificidae (1946), respectively.