Background: Persistent AIR leaks occur after Spontaneous pneumothorax both primary and secondary, and after lungs trauma and lung surgeries are sever problems encountered chest surgeons with. Persistent AIR leak causes longer patients hospitalization. Materials and Methods: We used autologous blood pleurodesis in patients with persistent AIR leak for 30patients with more than 8 days AIR leaks, during a three years period 1377-1380 (1999-2002). Results: The patients had 19 years up to 70 years old. Eight patients had thoracotomy and lobectomy and/or segmentectomies 6 with primary pneumothorax, 10 with secondary pneumothorax, and four with penetrated or blunt thoracic traumas. Blood was obtained from femoral or brachial veins and 70-150 mls. Injected in chest tubes. Chest bottle was first lied 80cm higher than body levels. After 24 hours repositioned in normal levels, and patients were supervised. Via chest tube we injected blood 70-100ml.for young patients, and 100-150 ml for older patients into intra pleural space. There were no clamped chest tubes. There were no pain, respiratory distress, fever, or cough in pleurodesized patients. The only patients complaint was local pain in femoral vein or brachial vein because blood sampling and blood obtaining, although there was no local visible complication as hematoma or bleeding. After 48 hours in 24 patients AIR leak ceased. In six patients because persistent AIR leak autologous blood pleurodesis repeated, two patients after 48hours AIR leak ceased, remaining four patients underwent for thoracotomies. success rate was 86.6%.Conclusion: According above success rate we suggest autologous blood pleurodesis in patients with persistent AIR leak is a reliable, effective, and no complicated procedure for persistent AIR leaks.