Background and Aims: Diabetes is one of the most common diseases in the world, which is responsible for approximately 4 million deaths per year. Due to the fact that in diabetes, the transfer and oxidation of glucose is impaired and the heart muscle cells get the energy they need exclusively from fatty acids, the products of fat oxidation, including free radicals in the cell. Increased cardiac output and oxidative stress. Oxidative stress with impaired prooxidant-antioxidant balance leads to impaired redox signaling and cellular and molecular damage to tissue components. Therefore, the use of antioxidants such as thistle extract may play an important role in reducing the consequences of diabetes. Because antioxidants with different mechanisms reduce the severity of oxidative stress reactions and cellular effects and ultimately reduce the clinical problems caused by them . The compounds in thistle extract may inhibit free radicals and their destructive effects; Therefore, prescribing Tribulus terrestris extract due to the antioxidant effects of this plant and also due to its effect in eliminating harmful metabolites in the body can be useful and effective in reducing the effects of oxidative damage . Other treatments for diabetics include exercise and physical activity. But you have to be very careful in choosing the intensity and type of sports activity. Because physical activity may cause oxidative stress in the body, which can activate enzymatic and non-enzymatic antioxidant components . Given the importance of the heart in life, the negative impact of diabetes on individual and social life on the one hand and the role of oxidative stress in diabetes on the other hand and that a study on the effect of aerobic exercise and prickly pear on heart enzymes Has not, the present study seems necessary. Methods: The present study was an experimental study. For this study, 45 mice aged 6 to 8 weeks in the weight range of 200 to 300 g after entering the research environment and one week of familiarity with the new environment, were randomly divided into six groups 1) Sham group (5 rats), 2) Healthy control group (8 rats), 3) Diabetic control group (8 rats), 4) Diabetic group Received 10 mg per kg of extracts of perch body weight per day (8 Rat head), 5) Diabetic group aerobic exercise (8 rats) 6) Diabetic group aerobic exercise and receiving thistle extract (8 rats) were divided. Then 32 rats were injected with 95 mg / kg body weight of mice dissolved in nicotinamide saline and after 15 minutes 55 mg / kg body weight of STZ diluted in sodium citrate buffer with pH = 7/4 became diabetic subcutaneously. Rats in control groups received the same amount of buffer. Five days after injection, rats with serum glucose above 300 mg / dL were considered diabetic using glucometry. Rats receiving thistle received 10 mg of thistle extract daily by gavage . After 5 days of familiarization, the exercise groups performed aerobic exercises on a treadmill at a speed of 23 meters per minute, 30 minutes per day, 5 days a week for eight weeks. The present exercise training protocol was implemented between 6. 00 and 8. 00 in the morning . Also, to stimulate the mice to run, an acoustic stimulus (impact on the wall of the treadmill) was used. During the protocol, the control mice were introduced to the treadmill, one session per week, for 5 minutes, at a speed of 10 meters in a minute and with a zero slope, they walked on the treadmill. After 6 weeks, all rats were anesthetized with respiratory chloroform and histoplasty was performed and MDA, GPX and SOD levels were measured. Finally, descriptive statistics and Shapiro-Wilk tests, one-way analysis of variance and Tukey post hoc using SPSS / 21 software were used to analyze the data. Results: In the present study, it was found that a period of aerobic training and itching had a significant effect on the MDA of heart tissue in Wistar rats (F5. 42 = 49. 85, p = 0. 001). It was also found that between the healthy-sham control group (p = 0. 712), the healthy control group with aerobic training and thistle training (p = 0. 672) and the sham group with aerobic training and thistle training (p = 0. 329). There is no significant difference, but there is a significant difference between other groups. Regarding GPX, in the present study, it was found that a period of aerobic training and diarrhea had a significant effect on GPX in the heart tissue of Wistar rats (F5, 42 = 85. 36, p= 0. 001). The results also showed that there was no significant difference between the healthy control group with the aerobic exercise group (p = 0. 715) and Kharkhask (p = 0. 184) on the one hand and the sham group with the diabetic control group (p = 0. 995) but There is a significant difference between the other groups. Regarding SOD, in the present study, it was found that a period of aerobic training and diarrhea had a significant effect on the SOD of the heart tissue of Wistar rats (F5, 42 = 245. 68, p = 0. 001). The results also showed a significant difference between the healthy control group with the aerobic exercise group (p = 0. 115) and aerobic exercise and thistle (P = 0. 948) on the one hand and the sham group with the diabetic control group (p = 0. 995). There is no but there is a significant difference between other groups. Conclusion: The results of the present study indicate the positive effect of aerobic exercise and thistle extract on the oxidative stress indices of heart tissue in diabetic rats. Various researches in this field have been carried out inside and outside the country and contradictory results have been obtained and different mechanisms have been proposed. But in general, it seems that in diabetic patients, regular moderate-intensity aerobic exercise can increase antioxidant capacity and increase resistance to oxidative factors . The first mechanism that affects the indicators of oxidative stress following exercise is exercise status (type, intensity and duration of exercise). The results of previous studies indicate the role of endurance training and adaptation to aerobic exercise in significantly reducing the oxidative pressure of the heart, which was associated with increased levels of antioxidant enzymes . Prolonged exercise counteracts this effect by increasing antioxidant enzymes and thus reducing the production of free radicals. Studies in mice have also shown that endurance training increases the levels of antioxidants and antioxidant enzymes in skeletal and cardiac muscle, thus providing protection against oxidative stress . Many studies on the effects of exercise on oxidative stress have reported that acute and endurance exercise reduces the oxidative stress damage caused by exercise.  On the other hand, studies have shown that inhibition of oxidative processes in diabetic patients may can reduce the incidence and spread of DELAYED complications in these patients; Therefore, supplementation with antioxidants can be a good way to reduce oxidative stress and its side effects . Also, the main chemical constituents of Tribulus terrestris include saponins, alkaloids, flavonoids, sterols and troestrosins E and A and the pharmacological effects of the fruit of this plant are attributed to these compounds. Researchers have shown that thrush can increase the level of non-enzymatic antioxidants in the body and intensify the activity of antioxidant enzymes and reduce lipid peroxidation . Tribulus terrestris saponins have been shown to reduce cell damage by activating the PKC signaling pathway . Also, some of the beneficial effects of this plant in the present study can be attributed to the effects of reducing its oxidative stress due to its antioxidant properties and strengthening the oxygen free radical scavenging system, which is probably very similar to vitamin E in this respect. The compounds in this plant purify various reactive oxygen species such as superoxide anion (O2-) and hydroxyl radical (OH) . According to the research results, the use of aerobic exercise can be recommended with confidence to reduce the negative effects of diabetes in diabetic rats, but in the case of the use of supplements, it is recommended to be taken in consultation with a physician.