Vinca (Catharanthus roseus) is a model medicinal plant. Roots of this plant is a place for accumulation of both alkaloids ajmalicine and serpentine. These materials are used for treatment of cardiovascular diseases, such high blood pressure. This plant is also very important, for production of alkaloids, vinblastine and vincristine, which are used in cancer treatment. Ethylene treatment significantly increased production metabolites of this plant. The objective of this research was investigation of ethylene effect on expression of some genes and detection of responsible genes for increase these metabolite. Effect of ethylene on the expression of T16H, G10H, DAT and AVLBS genes, at time course of 6, 12, 24, 48 and 72 hours, by Real time RT-PCR was investigated. Expression of T16H, DAT and AVLBS genes were up-regulated at 6 h after treatment with ethylene and down-regulated at 12 hours, then followed steady pattern. Also, the expression of G10H was increased at 72 h, and did not follow the pattern of the other three genes. According to results of the G10H gene is important gene to respond to ethylene.