Background: One of the most common methods for assessing cardiac damage is the measurement of biological indicators related to heart tissue damage. This study aimed to compare the effects of two types of acute weight loss programs (2 and 4%) on the levels of Creatine Kinase and troponin enzymes in the elite wrestlers. Materials and Methods: For this purpose, 16 young wrestlers (age: 18± 1. 31 years, weight: 71. 68± 13. 17 kg, height: 171± 6. 40 cm) in the form of crossover were randomly divided into two groups with weight loss of 2 and 4% of body weight. Blood samples were analyzed in four stages: before, immediately, 6 hours and 24 hours after losing weight. Repeated measures ANOVA and Bonferroni post hoc test for evaluation of within groups variations, and independent T-test for comparison between groups were used. Results: The results of this study showed that CK-MB was significantly increased in two groups after weight loss (P=0. 009). However, within 24 hours after weight loss, the level of isoenzyme Creatine Kinase returned to the level of resting. Also, there was a significant increase in cTnI level in the two groups (P=0. 004), this difference was more in the 2% than the 4% group, however, within 24 hours; weight loss was returned to resting levels. Conclusion: Although the level of CK-MB and cTnI enzymes increased in two groups after rapid weight loss, but rapid weight loss of 2 and 4% of body weight cannot cause serious cardiac damage.