Vincristine is an anti-cancer drug which fights the growth and spread of cancer cells in the body. This study aimed to investigate the protective effects of hydroalcoholic extract of chicory plant (Cichorium intybus)on hepatotoxicity of vincristine sulfate in rats. Adult male Wistar rats were used in this study. 56 mice were divided into 7 groups of eight. Treatments were intraperitoneally performed for 15 days. The control group contained healthy mice. Experimental groups 1 and 2, each contains three subgroups with defined concentrations: Experimental group a1 received 15 mg/kg of chicory extract, 0. 05 mg/kg of vincristine sulfate and again chicory extract. Experimental group b1 received 20 mg/kg of chicory extract, 0. 1 mg/kg of vincristine and chicory extract again. Experimental group c1 received 25 mg/kg of chicory extract,then, vincristine at a dose of 0. 2 mg/kg, and then chicory extract again. Experimental group a2: first 0. 5 mg/kg vincristine then chicory extract at 15 mg/kg dose, and received Wayne Christian. Experimental b2: First, they received 0. 1 mg/kg of vincristine,then, 15 mg/kg of chicory extract and again of vincristine. Experimental c2: first, they received 0. 2 mg/kg vincristine, then 15 mg/kg chicory extract, and again vincristine. After performing the above steps by anesthetizing the animals, blood sampling from mice, serum isolation, liver isolation, the measurement of the level of biochemical parameters, and liver enzymes, and their liver tissue were tested. In this study, a significant increase in ALT and AST enzymes was observed in experimental groups 1 compared to the control group, and also a significant decrease was observed in experimental groups 2 compared to experimental groups 1. The results showed that the subcutaneous injection of vincristine had additive effects on the number of liver enzymes in experimental group 1, the chicory extract had high antioxidant properties and significantly reduced liver enzymes.