CYCLOSPORINE A (CSA) is currently regarded as one of the common Immunosuppressive agents in organ transplantation among autoimmune patients and GVHD prevention after bone marrow transplantation. It exerts its effects through inhibition of (IL -2, 3, 4) queue expression and IL -2R IFN -r and GM - CSF. The monitoring of CSA is a useful approach to reduce the risk of graft rejection and to prevent its toxicity.This monitoring is performed to obtain optimal doses of CSA peak and exact levels in each patient. To assess the level of CSA in the blood, there are multi-technical methods such as HPLC, RIA, FPIA,... .In this study, CYCLOSPORINE - A (CSA) was conjugated bovine serum albumin (BSA) by glutaraldehyde chemical cross - linker .CSA - BSA conjugate was injected to rabbits and goats subcutaneously in order to obtain CSA specific anti - sera increase in antibody titer was achieved after two times injections.Goat and rabbit sera are detected casein - CSA in a titration of 1: 12000 and 1:8000 respectively. Hyper immune goat and rabbit sera were subjected to caprylic acid precipitation and IgG fraction was conjugated to horse radish peroxidas .These antibodies were able to detect standard levels of CSA (minimum amount =100 mg/ml) in an indirect Elisa assay .The findings suggest these antiserums to be used in the detection of CSA in RBC ,whole blood and patients sera by multi-technical methods, e.g: Immunohistochemistry , immunocytochemistry and Immunofleurescens in future.