In order to identify thylenchs species (Superfamily Tylenchoidea and suborder Aphelenchina) in Kerman province, 150 soil and root samples were collected from Kerman province during 2004 and 2005. The samples were washed and the nematodes extracted by centrifugal floatation technique. They were then fixed and transfered to glycerin according to the De Grisse method (1969). The permanent microscopic slides were prepared from the extracted nematodes. Morphological and morphometrical characters of the species were studied by light microscopy. As a resalt, 21 species belonging to several genera Tylenchoidea, three species of Aphelenchides and one species of Stictylus were identified. Among them, CEPHALENCHUS lobus Dhanachand & Jairajpuri, 1980, Coslenchus areolatus (Egunjobi, 1967) Siddiqi, 1978 and Rotylenchus eximius Siddiqi, 1964 are new records for Iran.

National Botanical Garden of Iran (NBGI) includes endemic and exotic plants that besides a source of plant genetics, it is considered as a wealthy source for research and education of botany in Iran. The plant parasitic nematodes in some collections including Hyrcanian habitat, Zagros habitat and Medicinal and aromatic garden were studied by collecting soil and root samples from trees, shrubs and annual plants. The nematodes were identified based on morphological and morphometric characters and based on these characters 34 species belonging to 19 genera were identified. Pratylenchus spp. with four species, followed by Helicotylenchus, Meloidogyne and Paratylenchus each one with three species were the more frequent genera. The species of Tylenchidae family showed higher frequency and multiplicity in Medicinal and aromatic collection. Due to infection of Brant’ s oak (Quercus brantii Lindl. ), oak manna (Q. infectoria Oliv. ), Pistacia atlantica and a few of medicinal plants to some species of plant parasitic nematodes i. e. Root Knot Nematodes, Root Lesion Nematodes, Ring and Dagger Nematodes, establishment of new plots with the seedlings free from the plant parasitic nematodes is emphasised. CEPHALENCHUS daisuce, Coslenchus capsici from Hyrcanian habitat and Paratylenchus ciccaronei from Medicinal and aromatic garden are new records from Iran.