Osteoporosis is a disease of high prevalence with increased bone loss. It has potential public health threat and that the health authorities had taken several measures for its control. In recent years, considerable attention has been given to physiopathology of osteoporosis. Oxidative stress has been proved to be involved in bone resorption. Oxidative stress occurs when balance between oxidants and biochemical antioxidants is disrupted because of excess reactive oxygen species.Thus, we measured CATALASE ACTIVITY, as one of the marker of stress oxidative, in 138 women. Participants were selected by inclusion and exclusion criteria from those who were referred to Jamie Clinic in Tehran for BMD evaluation. CATALASE antioxidant ACTIVITY was 262.01±44.70 k/gHb in the group of subjects with osteoporosis in comparison with the group of healthy subjects, 273.77±46.92 k/gHb. The results show that CATALASE ACTIVITY in patients with bone deficiency was less than in the control group, though it was not significant. This difference was more between control and patients group with more acute disease (T score -1.7) than patients group with milder disease (T score<-1).The results show that it may be useful to monitor osteoporosis in the more expanded sample size to obtain more definition results.

Background: Anembryonic gestation (blighted ovum) is the most common identifiable pathology in the first trimester of pregnancy, always leads to miscarriage. Early pregnancy failures from blighted ovum are often due to chromosomal abnormalities and a poor quality of sperm or egg. Oxidative stresses as a factor of disturbance balance between the production of free radicals and antioxidant defenses is involved in the pathogenesis of many diseases, including mouth and throat cancer and cardiovascular disease. CATALASE is one of the defensive systems against damages caused by oxidative stress in human. The aim of this study was to compare the ACTIVITY of salivary CATALASE in women with blighted ovum and women with history of normal pregnancy.Methods: This case-control study was performed on 34 patient women with blighted ovum and 34 healthy women as a control group. The study was performed in biochemistry laboratory at the University of Guilan from October 2015 to July 2015.The age range was 20-44 years and 18-45 years in patient and control groups, respectively. Unstimulated saliva samples were collected using spitting method.CATALASE ACTIVITY was measured by evaluating the constant rate of hydrogen peroxide decomposition in patient and control groups.Results: The patient group matched with healthy subjects in average age and having no other diseases history. The biochemical enzymatic assays indicate that the average CATALASE activities of saliva in patient and control groups were 14.47±3.8 and 16.42±3.48, respectively. Therefore, the CATALASE ACTIVITY was significantly reduced in patient group as compared to the control group (P=0.03).Conclusion: The obtained results suggested that oxidative stress plays an important role in the pathogenesis of blighted ovum. Therefore, determination the ACTIVITY of other antioxidant enzymes, in addition to catalse, may be used as a marker for diagnosis of blighted ovum. More studies with larger studied-population is recommended to confidently comment on the results of this study.

Background and Objectives: CATALASEs are a good scavenger of H O which degrades hydrogen peroxide into water and 2 2 oxygen. They are considered as a virulence factor that are present in both spores and hypha of fungi. There is limited data regarding CATALASE ACTIVITY in Aspergillus species. This study aimed to assess the mycelial CATALASE ACTIVITY of clinical and environmental isolates of Aspergillus niger, A. tubingensis, A. flavus, A. luchuensis, A. piperis and A. terreus. Materials and Methods: Briefly, clinical and environmental Aspergillus species were used in the current study. CATALASE ac-tivity was assessed for both groups of isolates including 13 A. flavus (12 clinical, 1 environmental), 13 A. terreus (8 clinical, 5 environmental), 26 A. tubingensis (13 clinical, 13 environmental), and 44 A. niger (25 environmental, 19 clinical) species. Fungal balls of mycelia were separated from the liquid culture and were crushed using homogenizer. The supernatants were collected and used for a CATALASE ACTIVITY assay. Results: Totally, in our study 98 Aspergillus including 45 environmental and 53 clinical isolates were assessed for CATALASE ACTIVITY. High CATALASE ACTIVITY was detected among environmental Aspergillus species (Mean= 1. 62 mU/ml) and the mean of mycelial CATALASE ACTIVITY among clinical A. terreus isolates was higher than environmental strains. Conclusion: In summary, mycelial CATALASE ACTIVITY varied among species and environmental isolates demonstrated higher CATALASE ACTIVITY. Totally a significant difference was found between clinical and environmental Aspergillus isolates.

Background: CATALASE enzyme plays an important role in the anti-oxidation defense of body so it is important to measure its ACTIVITY. Nowadays CATALASE ACTIVITY measurement is performed by expensive imported kits in various scientific fields. The purpose of this study was to design a sensitive fluorimetry method for measuring CATALASE ACTIVITY with improved sensitivity, accuracy and speed.Materials and Methods: In this study, the reaction of hydrogen peroxide with peroxidase (as a reaction accelerator) was used in fluorimetry for CATALASE ACTIVITY measuring in serum samples in order to increase the sensitivity of the assay. The sensitivity and intra- and inter-assay accuracy, verification test, recovery and parallelism tests, comparison method and correlation and coherence investigation methods were also performed. In order to increase the accuracy and speed of reading, the assay was performed in microplates and reading was done in fluorimetry plates.Results: The percentage of intra- and inter-assay variation coefficients were measured 3.8-6.6 % and 4.1-7.3%, respectively. Comparison of the results of mentioned method for 50 serum samples with common colorimetric method showed a good correlation (0.917). In assessing the accuracy, the recovery percent was obtained 91% to 107%. The test sensitivity was measured 0.02 IU/ml.Conclusion: The fluorimetry method by microplate reading has a sufficient precision, accuracy and efficiency for CATALASE ACTIVITY measuring as well as speed of measurement.Thus it can be an alternative method to conventional imported colorimetric methods.

Background: In hypothyroidism and hyperthyroidism, disturbance of oxidant/antioxidant balance leads to reactive oxygen species (ROS) generation. The aim of this study is assaying total antioxidant capacity and superoxide dismutase and CATALASE activities in patients with hypo-and hyperthyroidism in order to control the progression of its pathology and health care.Materials and Methods: This case-control study was performed on 85 patients with hypothyroidism, 66 patients with hyperthyroidism and 74 normal individuals as control that referred to the clinic of the Research Institute for Endocrine Sciences of Shahid-Beheshti University in year 2010. Serum enzymatic ACTIVITY of CATALASE, superoxide dismutase and total antioxidant capacity was measured in the fasting state. Data was described as mean±SD and data means of the two groups was compared by independent t -test. Data was analyzed by SPSS-18 application.Results: The total antioxidant capacity in individuals with hyperthyroidism decreased compared to healthy controls, but individuals with hypothyroidism compared to the healthy control group showed no significant difference. CATALASE and superoxide dismutase ACTIVITY in hypo-and hyperthyroidism were significantly increased compared with healthy controls (p=0.005).Conclusion: Decreasing of antioxidant capacity in hyperthyroid patients is probably because of increased production of free radicals. There was not observed significant difference in total antioxidant capacity in hypothyroid patients. Also in hypo-and hyperthyroidism patients, increasing of enzymes ACTIVITY is probably due to increasing of the production of ROS.

Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls. Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30. 7± 13. 2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29. 16± 11. 2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), CATALASE ACTIVITY, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’ s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test. Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0. 001). But there were no significant differences in CATALASE (P=0. 49) and total antioxidant capacity (P=0. 22) of TMD patients and healthy controls. Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.

Aims: Hyperglycemia is considered as the source of reactive oxygen species generation through glucose oxidation in diabetic patients. There are controversial reports on changes of total antioxidant capacity in diabetic patients. The aim of this study was to investigate the total antioxidant capacity and superoxide dismutase and CATALASE ACTIVITY as its components, in type II diabetic mellitus patients.Materials & Methods: This case-control cross-sectional descriptive study was performed during May-December 2009 in the Research Institute for Endocrine Sciences. 80 type II diabetic mellitus patients as the case group and 74 healthy volunteers as the control group were selected by easy given sampling method and their CATALASE and superoxide dismutase ACTIVITY as well as the total anti oxidation capacity was analyzed. Data was described as mean±SD and data means of the two groups was compared by independent T-test. Data was analyzed by SPSS 15 software.Results: The amount of total antioxidant capacity and CATALASE and superoxide dismutase ACTIVITY were significantly decreased in the diabetic group compared to the control group (p<0.05).Conclusion: The obtained data shows that antioxidant defense is decreased in type II diabetic patients. Oxidative stress and free radical generation probably play role in this situation and use of antioxidants can be studied in future as a solution.

Background: Regarding estrogen role in free radical scavenging, secretion cessation of estrogen in menopause is considered as the source of ROS increase. due to deficiency of estrogen, antioxidant defence system seems to be affected in this phase. The aim of this study was Assay of total antioxidant capacity and superoxide dismutase and CATALASE activities in menopausal women.Materials and Methods: This case-control study was performed on 75 postmenopausal women as a case group and 74 volunteer premenopausal women as a control was performed. Serum enzyme ACTIVITY of CATALASE, superoxide dismutase and total antioxidant capacity was measured in the fasting state.Results: The amount of total antioxidant capacity in postmenopausal (11.4±4.4 mM Trolox) compared to the control group (10.3±1.2 mM Trolox) were significantly decreased (p<0.05). The ACTIVITY of superoxide dismutase in postmenopausal (1.92±0.14 Unit/mg-protein) compared to the control group (2.07±0.09 Unit/mg-protein) were significantly decreased (p<0.05).Conclusion: The obtained data showed that total antioxidant capacity and ACTIVITY of superoxide dismutase enzymes decrease in the postmenopausal women. Increasing of Oxidative stress cause probably result in reduction of total antioxidant capacity and superoxide dismutase enzymes ACTIVITY.

In order to evaluate the effect of Fusarium verticillioides on seed yield, CATALASE (CAT) ACTIVITY on polyacrylamide gel and quantum yield of maize line, a factorial experiment based on randomized complete block design with five replications during 2017 growing season at the Payame Noor University, Mamaghan branch was performed. The experimental factors were two maize lines (B73 and MO17) and two levels of F. verticillioides (control and fusarium contamination). The results indicated that Fusarium verticillioides reduced seed yield, but enhanced CAT acivity in two maize lines. Further more, minimum fluorescence (Fo) in MO17 decreased, and increased in B73. Water splitting complex at donor side of PSII (FV/Fo) in B73 was increased, but was decreased in MO17. The level of maximum fluorescence (FM) in both line decreased. The energy necessary for closing reaction centers in PSII (Sm) and number of the QA reduction during fluorescence from Fo until FM (N) in MO17 were increased, but were decreased in B73. The results of this study revealed++ using from the chlorophyll a fluorescence can be a rapid and non-destructive method for evaluation of the maize reactions to injures caused by fusarium fungi infections.