Now a day, one of the new methods, using ionized gasses generated by cold atmospheric plasma (CAP) for the purpose of sterilization of dairy products. In this research, the effects of CAP on to inoculated milk andCANDIDA albicans were studied. Therefore, Sterilized milk with 3% fat, CANDIDA albicans fungi and CAP produced by dielectric barrier discharge (DBD) were used.CANDIDA albicans culturing was done in liquid Luria-Bertani (LB) medium and about 5.5× 105 colonies per milliliter of milk were inoculate. Then, the treatment of CAP on inoculated milk was done for 1, 3, 6, 9 and 12 minutes. In order to determine the CFU analysis, the treated samples were cultured on LB medium. Also, The variations of total content of free radicals and total antioxidant capacity (TAC) of milk after CAP treatment assayed by FRAP analysis, and Also lipid peroxidation of milk detected by evaluation malondialdehyde (MDA) using thiobarbituricacid. The results showed that by increasing the time of CAP treatment, the inactivity of fungi cells was enhanced. And, the minimum time required for sterilization of contaminated milk andCANDIDA albicansgrowth ceasing is 9 minutes. Generally, after this period of treatment time no growth was seen in the solid LB medium. As well as, comparing assayed levels of MDA and TAC between treated and control samples showed no significant differences. These results proposed that the CAP treatment could be appropriate way for sterilization ofCANDIDA albicans -contaminated cow milk.