In the developing world, the cancer as a prevalent cause of mortality is a new emerging challenges in medical and pharmaceutical sciences. Marine environs are regarded as a rich source of natural products with broad of therapeutic uses. Numerous BIOACTIVE PEPTIDES and depsi-PEPTIDES have been extracted from various marine organisms such as tunicates, sponges, molluscs and other marine organisms, with anti-cancer potential. They can produce the complex compounds which are more effective than presented anti-cancer drugs. Some of these marine PEPTIDES are under different clinical trials phases; they are secondary metabolites that produced by these organisms. According to different studies, their anti-cancer potential is related to some properties like antioxidant, anti- proliferative and anti-mutations effects. These PEPTIDES can stimulate cell death by various mechanisms, such as apoptosis, affecting the balance tubulin- microtubules (antimicrotubules), inhibition of angiogenesis, antiproliferative and cytotoxicity effects. Further studies on the reaction states of these compounds on cell cycle or apoptosis in cancer cells, are essential. The future of remedies, belong to the sea; and the sea, will have the major percentage in drug discovery, particularly in anti-cancer drugs.

In the fisheries industry, compared to other food industries, a large volume of byproducts such as bone, skin, and viscera are produced, which are costly to treat and dispose of in the environment. The rapid growth of tilapia and its marketability have also been considered. The value of proteins as the main source of amino acids in human health has been proven. In addition to their nutritional value, proteins have biological functions. These proteins show their functions through BIOACTIVE PEPTIDES. Production methods of bio PEPTIDES affect their biological activity. The articles showed that the use of enzymatic hydrolysis method for therapeutic applications makes them more stable than fermentation and solvent extraction methods and is a safer method than the other two methods. BIOACTIVE PEPTIDES have physiological effects such as antihypertensive, antioxidant, antimicrobial, anti-cancer, anti-diabetic, etc. and have many applications in the food, pharmaceutical and cosmetic industries and can be added to the food industry. The purpose of this article is to review the extraction of BIOACTIVE PEPTIDES from fish waste.

In this research, the optimization of protein hydrolysis process with the pancreatin enzyme was performed to evaluate the effect of time (30-270 min), temperature (20-60° C) and enzyme concentration (0. 25-3. 25%) on DPPH radical scavenging activity and Fe2+ chelating activity by using the response surface methodology. Optimum conditions to achieve the highest DPPH radical scavenging activity (42. 77%) and Fe2+ chelating activity (78. 57%), were obtained at time of 141. 1 min, temperature of 41. 93 ° C and enzyme concentrations of 2. 14%. Under these conditions, the degree of hydrolysis was 36. 8 ± 0. 35. Comparing the antioxidant power of the hydrolyzed protein and the intact protein showed that the hydrolysis increased the antioxidant activity of the fenugreek protein, significantly. Then, the antioxidant properties of optimum treatment were investigated at concentrations of 10-50 (mg/ml). The highest DPPH radical scavenging activity (50. 65%) and total antioxidant capacity (absorbance 1. 65 at wavelength of 695 nm), hydroxyl radical scavenging activity (69. 03%) and Fe2+ chelating activity (79. 72%) were achieved at concentration of 50 (mg/ml) and reducing power (absorption 0. 8 at wavelength of 700 nm) were achieved at concentration of 40 (mg/ml). The results showed that the hydrolysis of fenugreek protein with the pancreatin enzyme is an effective way to produce antioxidant PEPTIDES. So, the BIOACTIVE PEPTIDES, with significant antioxidant properties, can be used as potential substitute of synthetic antioxidants and as a protein supplement in food formulation to produce functional products with health promotion properties.