Background and Objective: Various techniques have been introduced to improve bond strength of zirconia which may negatively affect flexural strength. This study aims to evaluate the effects of different sandblasting parameters on micro-shear bond strength (MSBS, Mpa) and biaxial flexural strength (BFS, Mpa) of Zirconia. Methods: In this in vitro study, zirconia blocks were cut into 180 discs for MSBS and BFS tests (90 specimens in each group). For each test, they were divided into one control group and 8 experimental groups according to the type of sandblasting regimen (pressures of 4 or 6 bar, duration of 14 or 21 seconds, and alumina powder sizes of 50 or 110 μ, m, n=10). A universal testing machine was used to determine MSBS and BFS. X-ray diffraction (XRD) analysis was then performed. Findings: According to the results, groups with 110 μ, m particles (4 bar-14 s, 6 bar-14 s, 4 bar-21 s and 6 bar-21 s with MSBS values of 34. 43±, 5. 99, 35. 21±, 6. 39, 27. 17±, 3. 95 and 28. 66±, 3. 92 Mpa, respectively) had significantly higher MSBS values compared to the control group (p<0. 001, p<0. 001, p=0. 034, p=0. 005, respectively). Groups with 110 μ, m-21 s sandblasting regimen (with pressure of 4 and 6 bar with BFS values of 1031. 69±, 90. 00, 1062. 56±, 91. 29, 962. 30±, 93. 24, respectively) and those with 50 μ, m-6 bar-21 s sandblasting regimen had statistically significant lower BFS values compared to the control group (p<0. 001). According to XRD analysis, groups with 110 μ, m powder size and 21 s sandblasting resulted in more monoclinic phase. Conclusion: This study demonstrated that sandblasting with larger powder size and shorter duration could increase MSBS without any negative effects on BFS.