Due to the special medicinal benefits of the juice and mucilage of Aloe (Aloe vera L.), which are obtained from the leaves, today, these are widely used in pharmaceutical industries and cosmetics. Generally, Aloe can be propagatesd vegetatively but its propagation rate is too low. Therefore, a research was conducted to increase the rate of vegetative propagation and to obtain the best method of callus induction through in vitro culture of segmented leaves in tissue culture laboratory of Ramin Agricultural University of Ahwaz in 2004. The research was arranged in a completely randomized design. After surface sterilization, the leaf explants. were cultured aseptically in MS medium containing 3% sucrose and 0.8% agar supplemented with different concentrations and combinations of BAP, kinetin and 2,4-D, NAA and IBA for callus induction and shoot regeneration. The rate of callus induction and shoot regeneration were recorded after 4-5 and 12 weeks, respectively. Rooting media were MS medium containing 3% sucrose and 0.8% agar supplemented with different concentrations of NAA or IBA or without plant growth regulators. Root number, root length and root thickness and branching were compared between different rooting media after 6 weeks. Results showed that the basal portions of young leaves were suitable for callus induction. In this research the incidence of callus was better in darkness than in light. Actually, the rate of callus induction was very low in light. The combination of 2, 4-D and kinetin was the best for callus induction. Shoot regeneration was observed in MS medium containing BAP and combination of BAP and NAA or IBA. The best rooting medium was MS supplemented with 0.5 mg l-1 IBA.