The quality of nursing work life is an important index for assessing the professional health of nurses and providing tailored care services to patients. The study investigates the role of work-family conflict and selfregulation in predicting the quality of work-life among nurses. In this retrospective cross-sectional study, 230 nurses were selected randomly from public and private hospitals. They were asked to fill out Work-related Quality of Life Scale-2, Work-Family Conflict Scale, and self-regulation Questionnaire. Moreover, structural equation modeling was used to analyze the data by SPSS-19 and LISREL-8. 80 software. The results showed that the model had a good fit to the observed data (RMSEA=0. 06, GFI=0. 93, AGFI=0. 87, NFI=93, CFI=95, IFI=95, and P-value=. 073) and the final model was verified. The results also revealed that "behavior-based and time-based work-family conflicts", "assessing the plan's effectiveness", "searching for options", and "implementing the plan" could significantly predict "stress at work", "general well-being", "working conditions", employees' engagement", "job career satisfaction", and "work-home interference" (p<0. 05). It is indispensable to design tailored programs and professional health plans for improving family-work interference and self-regulatory actions, as two leading causes for the quality of nursing work life.