Introduction: Appropriate nutrition is an essential prerequisite for effective improvement of athletic performance, conditioning and recovery from fatigue after exercise, and avoidance of injury. Nutritional supplements containing carbohydrates, proteins, vitamins, and minerals have been widely used in various sporting fields to provide a boost to the recommended daily allowance. Coenzyme Q10 (CoQ10) is a naturally occurring, lipid-soluble compound that has been marketed for use by endurance athletes as an ergogenic aid. The aim of this study was to examine the effect of coQ10 dietary supplementation on athletics’ physiological factors such as: AEROBIC POWER, peak POWER, min POWER, average POWER and fatigue index.Materials and Methods: In this semi-experimental study, we studied 20men football players (age 30±2.22, weight 72.85±8.64 kg, height175.75±6.04cm, BMI23.50±1.90). Double-blind, placebo controlled method was employed. Players were divided randomly into two groups, oral CoQ10 group (DG) and placebo group (PG). Players in both groups performed RAST and Cooper test both before and after giving 100mg per day CoQ10 or placebo for 6 weeks to determine physiological factors such as AEROBIC POWER, peak POWER, min POWER, average POWER and fatigue index. We used paired t test and independent t test to analyze the data Independent t test were performed and p<0.05 were considered statistically significant.Results: DG group demonstrated improvements in AEROBIC endurance over the course of the study and there was significant difference (P=0.016) between the two groups. Supplementation with CoQ10 has no measurable effect on peak POWER (P=0.933), min POWER (P=0.258), average POWER (P=0.625) and fatigue index (P=0.569).Conclusion: Oral CoQ10 has measurable effect on AEROBIC endurance. The result of this study showed that at the end of the six week there was no difference between the two groups concerning peak POWER, min POWER, average POWER, and fatigue index.