Background and objectives: As a chronic inflammatory airway disease, asthma can increase level of markers of inflammation and muscle damage. In this study, we aimed to evaluate the effects of short-term continuous aerobic exercise combined with vitamin D supplementation on high-sensitivity C-reactive protein (hs-CRP), lactate dehydrogenase (LDH) and creatine kinase (CK) levels in asthmatic women. Methods: In this study, 30 overweight (body mass index=26. 97± 1. 24 kg/m2) women with mild to moderate asthma were purposefully selected. . The subjects received a vitamin D tablet with a daily dose of 1000 IU for six weeks. The training protocol consisted of two sessions of Balke treadmill test (one session before and one session after the vitamin D supplementation). Blood samples were taken before and immediately after the exercise and before and after vitamin D supplementation. Repeated measures ANOVA was used to evaluate changes in the levels of hs-CRP, CK and LDH. Results: The variables were not significantly affected by the exercise and vitamin D supplementation (P>0. 05). Conclusion: It seems that a single session of short-term aerobic exercise and six weeks of vitamin D supplementation do not significantly affect hs-CRP, CK and LDH levels in asthmatic women.