BIOFUELS DEMAND IS UNQUESTIONABLE IN ORDER TO REDUCE GASEOUS EMISSIONS (FOSSIL CO2, NITROGEN AND SULFUR OXIDES) AND THEIR PURPORTED GREENHOUSE, CLIMATIC CHANGES AND GLOBAL WARMING EFFECTS, TO FACE THE FREQUENT OIL SUPPLY CRISES, AS A WAY TO HELP NON-FOSSIL FUEL PRODUCER COUNTRIES TO REDUCE ENERGY DEPENDENCE, CONTRIBUTING TO SECURITY OF SUPPLY, PROMOTING ENVIRONMENTAL SUSTAINABILITY AND MEETING THE EU TARGET OF AT LEAST OF 10% BIOFUELS IN THE TRANSPORT SECTOR BY 2020.RENEWABLE, CARBON NEUTRAL, TRANSPORT FUELS ARE NECESSARY FOR ENVIRONMENTAL AND ECONOMIC SUSTAINABILITY. biodiesel DERIVED FROM OIL CROPS IS A POTENTIAL RENEWABLE AND CARBON NEUTRAL ALTERNATIVE TO PETROLEUM FUELS. THE IDEA OF ALGAE AS A RENEWABLE FUEL RESOURCE HAS BEEN INVESTIGATED SINCE THE 1950’S. BESIDES OIL, THE BULK OF ALGAL BIOMASS INCLUDES CARBOHYDRATES, MINERALS AND PROTEINS, WHICH CAN BE VALUABLE CO-PRODUCTS OF A biodiesel PRODUCTION PROCESS. THE AQUATIC SPECIES PROGRAM, WHICH WAS FUNDED BY THE US DEPARTMENT OF ENERGY, CARRIED OUT GROUNDWORK FOR ALGAE-TObiodiesel TECHNOLOGY. ONE OF THIS AQUATIC PLANTS IS AZOLLA AND WE USE IT FOR THIS PURPOSE. AZOLLA IS A GENUS OF FREE FLOATING AQUATIC FERNS WHICH IS PRESENTLY DISTRIBUTED THROUGHOUT TROPICAL AND TEMPERATE FRESH WATER ECOSYSTEMS. WE COLLECTED THEM FROM A SMALL RIVER IN BABOLSAR. WE EXTRACTED TRIGLYCERIDES BY SOXHLET EXTRACTOR. TRIGLYCERIDES ARE REACTED WITH METHANOL IN A REACTION KNOWN AS TRANSESTERIFICATION OR ALCOHOLYSIS. TRANSESTRIFICATION PRODUCES METHYL ESTERS OF FATTY ACIDS, THAT ARE biodiesel, AND GLYCEROL. IDENTIFICATION OF COMPOUNDS IN EXTRACTED SAMPLES WERE DONE BY GC/MS.