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Journal: MEDICAL SCIENCES JOURNAL OF ISLAMIC AZAD UNIVERSITY | Year:1391 | Volume:22 | Issue:3 (69) | Start Page:175 | End Page:183

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Title

GROWTH INHIBITORY AND DIFFERENTIATING EFFECTS OF ETHYL ACETATE SOLUBLE METABOLITE OF IRANIAN NATIVE BACTERIA, STREPTOMYCES CALVUS, IN HUMAN MYELOID LEUKEMIA K562 CELL LINE

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 Start Page 175 | End Page 183

Abstract

 Background: Because drug resistance is one of the most important problems in patients with CHRONIC MYELOID LEUKEMIA (CML), finding new anti-cancer drugs especially from natural sources is research priority. Therefore, in this study, anti-cancer effects of ethyl acetate soluble metabolite of Iranian native bacteria, STREPTOMYCES calvus, were studied using human CHRONIC MYELOID LEUKEMIA K562 cells.Materials and methods: In this experimental trail, ethyl acetate soluble metabolites were isolated fromS. calvus bacteria. K562 cell line was treated by various concentrations of this metabolite for 12-72h intervals. Anti-proliferative effects of ethyl acetate soluble metabolite were studied by trypan blue exclusion test. Wright-Giemsa staining and latex particle phagocytosis assay were used to study differentiated cells.Results: Ethyl acetate soluble metabolite induced growth inhibition in K562 cells in concentration and time- dependent manner. At the concentration of 200 ng/ml, the growth was inhibited 19-50% after 12-72h. Latex particle phagocytosis assay and Wright- Giemsa staining results revealed that K562 cells were differentiated toward monocytic- macrophagic lineage.Conclusion: According to growth inhibitory and differentiating effects of S.calvus metabolite in K562 cells, this metabolite can be proposed for more investigations in DIFFERENTIATION therapy of CML patients.

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