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Evaluation of Gamma Irradiation on Profile and Concentration of Organic Acids and Water Soluble Vitamins in Two Iranian Barberry Fruits


 Start Page 30 | End Page 44


High Performance Liquid Chromatography (HPLC)Q1


 Extracts of two varieties of Barberry including Berberis integerrima and Berberis vulgaris were prepared by water as a solvent, in darkness, with a pH=3 and at a temperature of 25˚ C. Then the effects of gamma radiation processing with doses of 0. 5, 1, 2. 5, 5, 7. 5 and 10 kGy were studied on the profile and concentration of Organic Acids and Water Soluble Vitamins. By a high-performance liquid chromatography (HPLC) in the control samples oxalic, tartaric, quinic, malic, ascorbic, shikimik, malonic, acetic, maleic, succinic and fumaric acids and C, B5, B6, B1, folic acid, biotin and B2 vitamins were identified. In the Berberis integerrima acetic, ascorbic and malonic acids and biotin, folic acid and thiamin vitamins; and in the Berberis vulgaris malic, ascorbic and malonic acids and B1, biotin and B5 vitamins were dominant. In both cultivars fumaric acid, in the Berberis integerrima B2; and in the Berberis vulgaris folic acid had the least amounts. After the gamma processing in the Berberis integerrima, Malic (10 kGy), acetic (0. 5 kGy) and ascorbic (10 kGy) acids and B5, biotin and B1 (0. 5 kGy) vitamins; and in the Berberis vulgaris acetic (0. 5 kGy), succinic (2. 5 kGy) and malic (7. 5 kGy) acids and B5 (10 kGy), B1 (1 kGy) and biotin (10 kGy) vitamins were the most.


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