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Information Journal Paper

Title

EVALUATION OF ALKALINE AND ACID PHOSPHATASE OF BLOOD AND MINERAL SUBSTANCE CHANGES DUE TO ULTRASONIC THERAPEUTIC EFFECT ON METACARPAL FRACTURE IN DONKEY

Pages

 Start Page 5 | End Page 18

Abstract

 The study was undertaken to evaluate enzymatic and MINERAL changes due to the effect of ULTRASOUND therapy after creating "Wedge FRACTURE" in the left metacarpal bone in 12 clinically healthy adult DONKEYs aged between 4 to 6 years and weighing 150 to 300 kg/BW. Animals were randomly divided into two groups of 6 animals each. These groups were further divided into two subgroups of Ia, IIa of one month and Ib, IIb of two months with 3 animals each.Ultrasound therapeutic regimen 1MHz and with intensity of 1W/Cm2 for 10 days was started a week after FRACTURE creation in animals of group II. Group I was considered as control.Intravenous blood was collected on days 0, 7, 14, 21, 28, 35, 42, 49, 56 and 60 for acid phosphatase, alkaline phosphatase, calcium, magnesium and phosphate changes in all animals of both groups by using calorimetric method. The enzymatic changes in both groups showed that alkaline phosphatase level in test groups was statistically significant at P<0.05 on days 14, 21, 28 and 30 in comparison with control. These changes with linear regression (slope) were more which indicated the rapid changes in animals of test groups. Acid phosphatase changes were observed on days 7 and 14 in the same pathern in both groups. Calcium and magnesium levels increased but phosphorus level decreased, which was not statistically significant. The little changes in calcium and phosphorus level were due to positive effect of ULTRASOUND in acceleration of callus formation at FRACTUREd site.

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