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Title

COMPARATIVE EVALUATION OF CYTOGENETIC ABERRATIONS INDUCED BY COBALT-60 IN GLIOMA CELLS IN THE PRESENCE OR ABSENCE OF IUDR

Pages

 Start Page 73 | End Page 84

Abstract

 Introduction: GLIOMA comprises about 50% of all primary central nervous system malignancies. Despite using surgery, radiotherapy and chemotherapy as means of treatment the prognosis for these patients remains poor. Efforts to improve the treatment of malignant GLIOMA include radiosensitization of cancer cells using Iododexyuridine (lUdR) as a thymidine analogue, which is preferentially incorporated into the DNA of tumor cells to sensitize them to radiation.Materials and Methods: In this study, we achieved Do for Glroma A 172 cell line in monolayer culture which was 1.7 Gy for exponentially growing cells and 2.2 Gy for plateau cells respectively. 1 mM IUdR alone induces significant CYTOGENETIC ABERRATIONS in A 172 glioblastoma cell line.  Results: The number of CYTOGENETIC ABERRATIONS induced by IUdR demonstrated a considerable discrepancy between exponentially growing and plateau phase cells. The value of sensitizing Enhancement Ratio (SER) derived through colony assay was equal to 1.36 for exponentially growing cells and 1.04 for plateau cells, whereas metaphase analysis showed 2.49 for exponentially growing cells and 1.05 for plateau phase cells. Discussion and Conclusion: Different findings of SER values between colony assay and metaphase analysis methods might be due to the repair time given to the cells during colony assay method. The difference in SER values between exponentially growing cells and plateau cells is probably due to different uptake of IUdR by cells in these two phases One of the main barriers for the effectiveness of IUdR when accompanied with external beam therapy is the presence of Go cells. As the drug alone induces aberrations, continuous long intra-arterial infusion of IUdR in place of intra-venouse infusion, may lead to better results.

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