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Information Seminar Paper

Title

CLONING AND EXPRESSION OF TRX TAGGED RECOMBINANT HUMAN GROWTH HORMONE(TRX-RHGH) IN CYTOPLASM OF ESCHERICHIA COLI AND PURIFICATION WITH NI-NTA COLUMN CHROMATOGRAPHY

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Abstract

 BACKGROUND AND AIM: HUMAN GROWTH HORMONE IS A POLYPEPTIDE WHICH CONSISTS OF 191 AMINO ACIDS AND TWO DISULFIDE BONDS WITH MOLECULAR WEIGHT OF 22KDA SECRETING BY HUMAN PITUITARY GLAND. THIS HORMONE NOT ONLY HAS MANY FUNCTIONS IN HUMAN BODY BUT IT ALSO HAS THERAPEUTIC APPLICATIONS. MANY HOSTS HAVE BEEN USED FOR EXPRESSING AND PRODUCING OF RECOMBINANT HUMAN GROWTH HORMONE (RHGH) BUT E.COLI IS THE PREFERRED HOST. ...

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