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Title

PREVALENCE OF GES, PER AND TEM GENES (ESBLS) IN ACINETOBACTER BAUMANNII CINICAL ISOLATES IN ICU SECTION OFQAEM HOSPITAL –MASHHAD

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Abstract

 BACKGROUND AND AIM: ACINETOBACTER BAUMANNII IS AN IMPORTANT OPPORTUNISTIC PATHOGEN THAT MAINLY INFECTS CRITICALLY ILL PATIENTS IN INTENSIVE CARE UNITS. THE PRODUCTION OF PLASMIDE-MEDIATED EXTENDED- SPECTRUM B -LACTAMASES (ESBLS) IS ONE OF THE MOST IMPORTANT MECHANISMS OF RESISTANCEAGAINST B -LACTAM ANTIBIOTICS. THIS STUDY WAS DONE FOR DETECTION OF THE PREVALENCE OF ESBL TYPE GES, PER AND TEM IN ACINETOBACTER BAUMANNII CINICAL ISOLATES IN ICU QAEM HOSPITAL –MASHHAD METHODS: IN THIS DESCRIPTIVE STUDY, A TOTAL OF 140 A. BAUMANNII ISOLATES WERE RECOVERED FROM HOSPITALIZED PATIENTS IN ICU QAEM HOSPITAL IN MASHHAD CITY BETWEEN DECEMBER 2012 AND MARCH 2013. IDENTIFICATION OF A. BAUMANNII ISOLATES WAS CARRIED OUT BY USING MICROBIOLOGICAL AND BIOCHEMICAL LABORATORY METHODS AND THEN CONFIRMED BY USING OXA-51 PCR SCREENING. ALL OF THE A. BAUMANNII ISOLATES WERE SCREENED FOR PRODUCTION OF ESBLS BY COMBINATION DISK TEST. THE PREVALENCE OF BLAPER, BLAGES, BLATEM, WAS DETERMINED BY PCR METHOD USING SPECIFIC PRIMERS….

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