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Title

PCR DETECTION OF BLA-SHV RESISTANCE GENE IN ESCHERCHIA COLI ISOLATED FROM MILAD HOSPITAL

Writers

MIRZAIE PARSA SHIMA | SOLTANI BANAVANDI MOHAMMAD JAVAD | SHAHHOSSEINY MOHAMAD HASAN | RAHBAR MOHAMMAD

Pages

 Start Page | End Page

Abstract

 BACKGROUND AND AIM: HOSPITAL INFECTIONS CAUSED BY ANTIBIOTIC RESISTANCE BACTERIA ARE INCREASING IN THE WORLD. RESISTANCE TO EXTENDED-SPECTRUM BETA-LACTAMASE (ESBLS) IS ONE OF THE MOST IMPORTANT ANTIBIOTIC RESISTANCE MECHANISMS. ACCORDING TO THE PREVALENCE OF E.COLI PRODUCING ESBL, WE ATTEMPTED TO IDENTIFY THE BLASHV GENE WHICH IS ONE OF THE CAUSING GENES OF THE RESISTANCE TO BETA – LACTAM ANTIBIOTICS BY PCR METHOD.METHODS: 100 ISOLATES OF E.COLI WERE COLLECTED FROM MILLAD HOSPITAL IN TEHRAN. ANTIBIOGRAM TEST WAS DONE AND ESBL POSITIVE SAMPLES WERE DETERMINED BY COMBINATION DISK TEST. THEN, DNA EXTRACTION OF SAMPLES WAS CARRIED OUT AND THE PRESENCE OF BLASHV GENE WAS EVALUATED BY USING PCR METHOD. LIMIT OF DETECTION (LOD) AND SPECIFICITY TESTS WERE SURVEYED AND PCR PRODUCT WAS CLONED IN T/A VECTOR…..

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