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Title

MOLECULAR DETECTION OF METALLO- Β -LACTAMASE GENES, BLAIMP-1, BLAVIM-2 AND BLASPM-1 IN IMIPENEM RESISTANT PSEUDOMONASAERUGINOSA ISOLATED FROM CLINICAL SPECIMENS IN TEACHING HOSPITAL OF MAHABAD, IRAN

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Abstract

 BACKGROUND AND AIM: CARBAPENEM RESISTANT PSEUDOMONAS AERUGINOSAIS ARE A SERIOUS CAUSE OF NOSOCOMIAL INFECTIONS. THE MAIN PURPOSE OF THE STUDY WAS TO DETERMINE THE PREVALENCE RATE OF IMIPENEM RESISTANT PSEUDOMONAS AERUGINOSA CARRYING METALLO- BETA- LACTAMASE (MBL) GENES.METHODS: 145 PSEUDOMONAS AERUGINOSA ISOLATES WERE COLLECTED FROMTEACHING HOSPITAL OF MAHABADDURING A PERIOD OF 9 MONTHS IN 2013. THESE STRAINS WERE IDENTIFIED USING CONVENTIONAL MICROBIOLOGICAL TESTS. THE SUSCEPTIBILITY OF ISOLATES TO ANTIBIOTICS WAS ASSESSED USING DISK DIFFUSION TEST. THE IMP-EDTA COMBINATION DISK PHENOTYPIC TEST WAS PERFORMED FOR DETECTION OF MBL PRODUCING STRAINS. FINALLY, POLYMERASE CHAIN REACTION (PCR) WAS PERFORMED TO DETECT MBL GENES, BLAIMP-1, BLAVIM-2 AND BLASPM-1 IN IMIPENEM RESISTANT STRAINS…..

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