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Information Seminar Paper

Title

USING SINGLE TUBE MULTIPLEX ARMS/RFLP IN RECOGNITION OF THE MOST COMMON B GLOBIN POLYMORPHISMS

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Abstract

 INTRODUCTION: B- THALASSEMIA IS AN AUTOSOMAL RECESSIVE DISEASE THAT INVOLVE THE DECREASED AND DEFECTIVE PRODUCTION OF HEMOGLOBIN. FOR PND WE USED DIRECT DETECTION WITH AMPLIFICATION REFACTORY MUTATION SYSTEM (ARMS) AND INDIRECT DETECTION BY USING POLYMORPHISM MARKERS (RFLP) WITH RESTRICTION ENZYMES. IN THIS STUDY WE DEVELOPED A SINGLE – TUBE MULTIPLEX – PCR FOR ARMS/RFLP, FASTER AND LESS EXPENSIVE METHOD INSTEAD ARMS/RFLP METHOD FOR THREE SITES RFLPS …

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