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Information Seminar Paper

Title

MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS SPICES WITH IS6110-RFLP BETWEEN PATIENTS

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Abstract

 INTRODUCTION: MYCOBACTERIUM TUBERCULOSIS IS THE ONE OF THE HARMFUL HUMAN PATHOGENS IN THE WORLD AND NOW IS6110 –RFLP TYPING REMAINS THE INTERNATIONAL ACCEPTED STANDARD AND CONTINUES TO PROVIDE NEW INSIGHTS IN THE EPIDEMIOLOGY OFMYCOBACTERIUM TUBERCULOSIS INFECTIONS. GENETIC BASEMENT OF IS6110 IS BECAUSE OF DIFFERENT COPIES AND PLACES INDIFFERENT SPICES.MATERIAL AND METHOD: FIRST STAGE IN THIS TECHNIQUE IS THE SELECTION OF PROBE. THE IS6110 DNA PROBEWAS PREPARED WITH AMPLIFICATION OF 245 BP-FRAGMENT USING PCR.THIS FRAGMENT WAS PURIFIED AND LABELED BYDIGOXIGENIN.AFTER EXTERACTION OF DNA DO STAGE OF RFLP THEN DO STAGE OF HYBRIDATION RFLP PRODUCT WITH DIGOXIGENINPROB AT LAST ANALIZIS THE PATERN OF BANDS WITH GEL COMPAII. ...

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