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Information Seminar Paper

Title

GONAD TISSUE ENGINEERING BY ENCAPSULATING DIFFERENTIATED GERM CELL-LIKE CELLS DERIVED FROM HUMAN ENDOMETRIAL STEM CELLS (HENSCS) IN FIBRIN SCAFFOLD

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Abstract

 RECENT STUDIES IN STEM CELLS DIFFERENTIATION INTO GERM CELL-LIKE CELLS HAS FOCUSED ON INFERTILITY TOPICS AND REPRODUCTIVE PROBLEMS. HIGH POTENCY OF TUMOROGENICITY AND ETHICAL ISSUES OF EMBRYONIC STEM CELLS FOR CLINICAL TRIALS, HAVE LIMITED THEIR APPLICATIONS. INSTEAD, PLURIPOTENT CELLS ISOLATED FROM ADULT TISSUES OR ORGANS COULD BE A GOOD ALTERNATIVE FOR GAMETE PRODUCTION. WE IDENTIFIED THE SUITABLE CONDITIONS WHICH PROMOTED HUMAN ENDOMETRIAL STEM CELLS (HENSCS) DIFFERENTIATION INTO GERM CELL-LIKE CELLS BY REGULATING THE CONCENTRATION OF RETINOIC ACID (RA) IN 2D MEDIUM. AFTERWARDS, THE DIFFERENTIATED GERM CELL-LIKE CELLS WERE CULTURED IN A 3D FIBRIN SCAFFOLD AND THEIR VIABILITY AND PROPERTIES WERE EVALUATED.

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