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Information Seminar Paper

Title

CYTOTOXIC EFFECTS OF LECTIN FROM ELDERBERRY BARK ON CELL LINE FROM PUPA FAT BODY TISSUE OF COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA

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Abstract

 SAMBUCUS NIGRAL-AGGLUTININ-I REFERRED TO AS SNA-I IS A CHIMERIC LECTIN COMPOSED OF AN A-CHAIN WITH ENZYMATIC ACTIVITY AND A B-CHAIN WITH CARBOHYDRATE-BIDING ACTIVITY. BCIRL-LEPD-SL1 (CPB) CELL LINE ESTABLISHED FROM PUPA FAT BODY OF THE COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA (COL.CHRYSOMELIDAE). TREATMENT OF INSECT PUPAL FAT BODY CELLS WITH SNA-I CAUSED A LOSS OF CELL VIABILITY AFTER FOUR DAYS OF INCUBATION WITH DIFFERENT CONCENTRATIONS OF SNA-I (0.1- 30 MG/ML) BASED ON THE MTT TEST. IT WAS CLEAR THAT SNA-I HAD A STRONG GROWTH INHIBITORY EFFECT ON CPB CELLS. INCREASED CYTOTOXICITY WAS OBSERVED IN SNA-I TREATED CELLS IN A DOSE AND TIME DEPENDENT MANNER.

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