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Information Seminar Paper

Title

THE RESTORATIVE AND ANTI-INFLAMMATORY EFFECTS OF AQUATIC EXTRACT OFCANNABIS SATIVA LEAVES ON SPINAL GLIAL AND ALPHA-NEURONS FOLLOWING CENTRAL DEGENERATION IN RATS

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Abstract

 OBJECTIVE: MEDICINAL HISTORY OFCANNABIS SATIVA GOES BACK TO BC. THE PRESENT STUDY IS AIMED TO INVESTIGATE RESTORATIVE AND ANTI-INFLAMMATORY EFFECTS OF AQUATIC EXTRACT OF CANNABIS SATIVA LEAVES ON SPINAL GLIAL AND ALPHA-NEURONS FOLLOWING CENTRAL DEGENERATION IN RATS.METHODS: THIS EXPERIMENTAL STUDY WAS CONDUCTED ON 32 MALE WISTAR RATS. ANIMALS WERE DIVIDED INTO 4 GROUPS; A: CONTROL GROUP, B: COMPRESSION, C: COMPRESSION+TREATMENT WITH A DOSE OF 25 MG/KG, AND D: COMPRESSION+TREATMENT WITH A DOSE OF 50 MG/KG. AFTER SCIATIC NERVE COMPRESSION IN TREATMENT GROUPS B, C, AND D, EXTRACT INJECTION WAS DONE INTRA-PERITONEAL IN GROUPS C AND D WITHIN 2 WEEKS (ONCE A WEEK). 28 DAYS AFTER COMPRESSION, LUMBAR SPINAL CORD WAS SAMPLED AND AFTER TISSUE PROCESSING, CUTTING, COLOURING WITH TOLUIDINE BLUE AND PREPARING PHOTOS FROM SPINAL ANTERIOR HORN USING A PHOTOMICROSCOPE, NEURONAL AND GLIAL DENSITY OF EACH GROUP WAS CALCULATED BY DISSECTOR METHOD. FINALLY DATA WAS ANALYSED USING ANOVA AND TUKEY TESTS…..

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