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Title

PHYSIOLOGICAL AND MORPHOLOGICAL RESPONSES SEEDLING OF THREE BROMUS SPECIES TO WATER DEFICIT STRESS

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Abstract

 THE EFFECT OF DROUGHT STRESS WAS STUDIED ON SEED CHARACTERISTICS INCLUDING: PERCENT OF GERMINATION, SEEDLING LENGTH (MM/PLANT), ROOT/SHOOT LENGTH RATIO, SEEDLING WEIGHT (G), SEEDLING DRY/FRESH WEIGHT RATIO (G/DFW) AND SEED VIGOR INDEX (VI) IN THREE SPECIES OF BROMUS GENUS (BR.TOMENTELLUS, BR.INERMIS, BR.PERSICUS) IN GERMINATOR AND GREENHOUSE CONDITION. IN GREENHOUSE EXPERIMENT, THREE MORE PHYSIOLOGICAL CHARACTERISTICS AS CHLOROPHYLL, CARBOHYDRATES AND PROLINE CONTENTS WERE MEASURED. THE DROUGHT TREATMENTS WERE FOUR LEVELS OF OSMOTIC POTENTIAL (0, -0.3, -0.6 AND -0.9 MPA) IN GERMINATOR AND FOUR LEVELS OF OSMOTIC POTENTIAL (FC, 25% FC, 50% FC AND 75% FC) IN GREENHOUSE THAT WERE MADE BY POLY ETHYLENE GLYCOL (PEG 6000) SOLUTION AND FIELD CAPACITY METHOD, RESPECTIVELY. WITH REGARD TO RESULTS, FOR ALL SEED CHARACTERISTICS LORESTAN (9507) SPECIES WAS SUPERIOR COMPARED TO OTHER ECOTYPES. INCREASING OSMOTIC STRESS DECREASED THE CHLOROPHYLL CONTENT AND INCREASED PROLINE AND CARBOHYDRATE CONTENT. THE PHENOTYPIC CORRELATION BETWEEN MEASUREMENTS OF TWO CONDITIONS WAS DETERMINED. THE ESTIMATES WERE SIGNIFICANT FOR MOST TRAITS. RESULTS OF PROBIT ANALYSIS OF LD50 AND LD90 THE SAME TREND WERE OBTAINED.

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