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Title

DETECTION OF FUSARIUM LANGSETHIAE AND F. SPOROTRICHIOIDES DNA IN WHEAT

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Abstract

 INFORMATION CONCERNING THE TEMPERATURE REQUIREMENTS OF THE SPECIES CAUSING FUSARIUM HEAD BLIGHT OF SMALL GRAINS IS ESSENTIAL FOR UNDERSTANDING WHICH SPECIES CAUSE THE DISEASE IN DIFFERENT AREAS AND YEARS, FOR DEVELOPING WEATHER-DRIVEN DISEASE MODELS, AND FOR PREDICTING QUANTITY IN KERNELS.F. SPOROTRICHIOIDES DEVELOPED MORE FUNGAL BIOMASS THAN F. LANGSETHIAE IN DURUM WHEAT SPIKES OVER DIFFERENT TEMPERATURE AFTER INOCULATION HAD OCCURRED AT FULL ANTHESIS. F. SPOROTRICHIOIDES WITH 3.32 NG PER 50 NG TOTAL DNA COLONIZED THE SPIKES 3.7 TIMES MORE THAN F. LANGSETHIAE.THE OPTIMAL TEMPERATURE FOR F. LANGSETHIAE COLONIZATION WAS 25°C; LOWER QUANTITIES OF F. LANGSETHIAE DNA WERE DETECTED AT 10 TO 20°C, 30°C, AND 35°C, BUT NO F. LANGSETHIAE DNA WAS DETECTED AT 40°C. THE OPTIMAL TEMPERATURE FOR F. SPOROTRICHIOIDES COLONIZATION WAS 30°C, BUT COMPARABLE AMOUNTS OF DNA WERE PRODUCED BETWEEN 20 AND 35°C; LOWER QUANTITIES OF F. SPOROTRICHIOIDES DNA WERE DETECTED AT LOWER AND HIGHER TEMPERATURES, AND A VERY LOW QUANTITY WAS DETECTED AT 40°C.THE QPCR ASSAY PROVED TO BE A USEFUL TOOL TO DETECT THE DYNAMIC OF FUNGAL INVASION IN PLANTAAFTER INFECTION HAD OCCURRED, AND TO SINGLE OUT THE PRESENCE OF INFECTION BEFORE ONSET OF THE DISEASE SYMPTOMS. A ROBUST DETECTION OF THE INFECTION OCCURRED WITHIN 24-48 H FOR F. SPOROTRICHIOIDES AND WITHIN 2–4 DAYS FOR F.LANGSETHIAE. THIS RESULT IS VERY PROMISING FOR HIS POTENTIAL IMPACT ON TACTICAL DISEASE MANAGEMENT: A DETECTION OF INFECTION OCCURRENCE WITHIN 2 OR 3 DAYS MAY RESULT IN AN EFFICIENT APPLICATION OF CURATIVE FUNGICIDES.

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