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Title

HYDROQUINONE DIPHOSPHATE/AG+ AS AN ENZYMATIC SUBSTRATE FOR ALKALINE PHOSPHATASE CATALYZED SILVER DEPOSITION

Author(s)

 PEREIRA DA SILVA NEVES MARTA MARIA | GONZALEZ GARCIAA MARIA BEGONA | SANTOS DAVID HERNANDEZ | FANJUL BOLADO PABLO

Pages

  -

Keywords

Not Registered.

Abstract

 SIGNAL AMPLIFICATION BY ENZYME LABELS SUCH AS ALKALINE PHOSPHATASE (AP) USED IN AFFINITY ASSAYS (IMMUNO OR DNA BASED ASSAYS) IS AUSUAL STRATEGY TO GET HIGH SENSITIVE DETECTION METHODOLOGIES. AN ADVANTAGEOUS COMBINATION OF AN ENZYME SUBSTRATE HYDROQUINONE DIPHOSPHATE (HQDP) WITH SILVER IONS (AG+) IN SOLUTION IS PRESENTED IN THIS WORK AS A NEW ENZYMATIC SUBSTRATE FOR AP. THIS ENZYME CATALYZES THE DEPHOSPHORYLATION OF HQDP PRODUCING HYDROQUINONE, A REDUCING AGENT FOR SILVER IONS PRESENT IN SOLUTION.THUS BY MIXING AP, HQDP AND AG+, METALLIC SILVER IS DEPOSITED WHERE THE ENZYMATIC REACTION TAKES PLACE. A QUALITATIVE ASSAY IS PRESENTED IN NITROCELLULOSE MEMBRANES TO DEMONSTRATE THIS MECHANISM. THE AFFINITY REACTION BETWEEN BIOTIN AND STREPTAVIDINLABELLED WITH AP IS ALSO FOLLOWED BY THE ELECTROCHEMICAL DETECTION OF SILVER DEPOSITED ON THE SURFACE OF SCREEN PRINTED CARBON ELECTRODES. ANODIC STRIPPING VOLTAMMETRY OF ENZYMATICALLY DEPOSITED SILVER IS SHOWED AS A SENSITIVE DETECTION TECHNIQUE FOR BIOSENSING APPLICATIONS.

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    • Cite

    APA: Copy

    Pereira Da Silva Neves, Marta Maria, Gonzalez Garciaa, Maria Begona, Santos, David Hernandez, & Fanjul Bolado, Pablo. (2015). HYDROQUINONE DIPHOSPHATE/AG+ AS AN ENZYMATIC SUBSTRATE FOR ALKALINE PHOSPHATASE CATALYZED SILVER DEPOSITION. ANNUAL ELECTROCHEMISTRY SEMINAR OF IRAN. SID. https://sid.ir/paper/919867/en

    Vancouver: Copy

    Pereira Da Silva Neves Marta Maria, Gonzalez Garciaa Maria Begona, Santos David Hernandez, Fanjul Bolado Pablo. HYDROQUINONE DIPHOSPHATE/AG+ AS AN ENZYMATIC SUBSTRATE FOR ALKALINE PHOSPHATASE CATALYZED SILVER DEPOSITION. 2015. Available from: https://sid.ir/paper/919867/en

    IEEE: Copy

    Marta Maria Pereira Da Silva Neves, Maria Begona Gonzalez Garciaa, David Hernandez Santos, and Pablo Fanjul Bolado, “HYDROQUINONE DIPHOSPHATE/AG+ AS AN ENZYMATIC SUBSTRATE FOR ALKALINE PHOSPHATASE CATALYZED SILVER DEPOSITION,” presented at the ANNUAL ELECTROCHEMISTRY SEMINAR OF IRAN. 2015, [Online]. Available: https://sid.ir/paper/919867/en

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