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Title

APPLICATION EFFECT OF DIAZINON ON N-FIXING BACTERIA OF AZOSPERILLIUM GENUS

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Abstract

 THE GENUS OF AZOSPERILLIUM IS ONE OF THE MOST IMPORTANT FREE LIVING N-FIXING BACTERIA WHICH ARE FOUND IN ALMOST ANY TYPE OF SOIL. THESE BACTERIA HAVE GREAT ROLE IN THE CONVERSION OF INORGANIC NITROGEN (N2) TO ORGANIC ONE (-NH3) AND IMPROVE PLANT GROWTH. VARIOUS FACTORS CAN AFFECT NORMAL GROWTH OF THESE BACTERIA IN SOIL [1]. PESTICIDES ARE ONE OF THE MOST IMPORTANT FACTORS IN DISTURBING NATURAL ECOSYSTEMS [2]. THIS STUDY WAS AN ATTEMPT TO EXAMINE THE INFLUENCE OF DIFFERENT DOSE OF DIAZINON’ AS ONE OF THE MOST USED PESTICIDES, ON THE GROWTH OF THE AZOSPERILLIUM IN-VITRO CONDITIONS. FIELD RECOMMENDED DOSE FOR THIS PESTICIDE IS 1000PPM, THEREFORE THE EFFECT OF FIVE DIFFERENT DOSES OF DIAZINON (250, 500, 1000, 2500 AND 5000PPM) ON THE BACTERIAL ISOLATE WERE TESTED IN LABORATORY CONDITION. THE RESULTS SHOWED THAT THIS PESTICIDE HAS A SIGNIFICANT DECREASE IN BACTERIAL GROWTH EVEN AT THE LOWEST DOSE (250PPM), BUT IT IS NOT FULLY STOPPING THE GROWTH OF BACTERIA. AT THE RECOMMENDED DOSE FOR GARDENS AND FARMS (1000PPM) AS WELL AS THE HIGHER DOSES, THIS PESTICIDE CAN BE TOTALLY LETHAL FOR BACTERIA AND CAN FULLY DESTROY BACTERIAL POPULATION IN THE SOIL.

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