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Information Seminar Paper

Title

OPTIMIZING NUTRIENTS REQUIRED FOR MICROBIAL REMEDIATION OF AN OIL-CONTAMINATED DRILLING MUD PIT IN KHANGIRAN DISTRICT

Pages

 Start Page | End Page

Abstract

 A FIELD PROJECT FOR BIOREMEDIATION OF AN ABANDONED DRILLING MUD PIT WHICH WAS CONTAMINATED WITH GASOIL HAS BEEN PLANNED. IN ORDER TO OPTIMIZE AND EVALUATE THE INFLUENCE OF NITROGEN AND PHOSPHORUS CONCENTRATIONS ON THE BIODEGRADATION OF HYDROCARBONS BY INDIGENOUS MICROFLORA OF THE SOIL, EXPERIMENTS WERE DESIGNED BASED ON FULL FACTORIAL APPROACH. THE INDEPENDENT VARIABLES NAMELY NITROGEN CONCENTRATION (N) AND PHOSPHORUS CONCENTRATION (P) WERE INVESTIGATED IN FOUR LEVELS AND THE REMOVAL OF OIL AND GREASE (O& G) WAS MEASURED AFTER 60 DAYS OF BIOTREATMENT AS THE DEPENDENT VARIABLE. STATISTICAL ANALYSIS OF THE RESULTS SHOWED THAT PHOSPHATE AMENDMENT DID NOT HAVE PROFOUND EFFECT ON THE ACHIEVED BIODEGRADATION AND HENCE WAS NEGLECTED BASED ON ECONOMIC CONSIDERATIONS. CONVERSELY BIODEGRADATION EXTENT WAS SIGNIFICANTLY INFLUENCED BY NITROGEN ADJUSTMENT IN A WAY THAT A SHARP INCREASE IN OIL REMOVAL WAS OBSERVED BY INCREASING NITROGEN SOURCE FROM 0 PPM TO 250 PPM. HOWEVER, SUPERFLUOUS NITROGEN ADDITION UP TO 500 PPM REVEALED INHIBITORY EFFECT ON MICROBIAL ACTIVITY. ACCORDING TO THE OBTAINED RESULTS, THE SUPPLEMENTATION OF 250 PPM OF NITROGEN SOURCE IN WHICH OIL AND GREASE REMOVAL REACHED 63.7% WAS INDICATED AS THE OPTIMIZED CONDITION FOR THE FIELD PROJECT.

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