Seminar Paper

Paper Information

video

sound

Persian Version

View:

1,122

Download:

Cites:

Information Seminar Paper

Title

FULL AUTOMATED HOLLOW FIBER LIQUID PHASE MICROEXTRACTION FOR DETERMINATION OF MEGESTROL ACETATE AND LEVONORGESTREL BASED ON TWO IMMISCIBLE ORGANIC SOLVENT

Pages

 Start Page | End Page

Keywords

Not Registered.

Abstract

 MEGESTROL ACETATE IS A SYNTHETIC DERIVATIVE OF A NATURALLY OCCURRING PROGESTATIONAL AGENT, WHICH IS CHEMICALLY SIMILAR TO PROGESTERONE. THIS DRUG IS PRIMARILY USED AT HIGH DOSAGE FOR THE TREATMENT OF METASTATIC BREAST CANCER. LEVONORGESTREL IS ALSO A SYNTHETIC PROGESTATIONAL HORMONE. NORGESTREL WAS THE FIRST PROGESTATIONAL HORMONE WITCH SYNTHESIZED IN THE 1950S AS A RACEMIC PRODUCT AND LEVONORGESTREL IS THE BIOLOGICALLY ACTIVE LEVOROTORY ENANTIOMER OF NORGESTREL (D-NORGESTREL). IT IS USED AS A CONTRACEPTIVE AGENT AT LOW DOSES EITHER ALONE OR IN COMBINATION WITH ESTROGEN AS AN ORAL PILL [1]. PRESENCE OF THESE FREE DRUGS AND THEIR METHABOLITES IN BIOLOGICAL SAMPELS SUCH AS URINE AND PLASMA CAN SHOW THE PERCENTAGE OF DRUGS THAT WOULD BE EFFECTIVE AS A PHARMACEUTIC SUBSTANCE, HOWEVER, THIS IS NECESSARY THAT WE CAN DETECT THE AMOUNTS OF FREE DRUGS IN BIOLOGICAL SAMPLES. IN THIS RESEARCH AN AUTOMATED INSTRUMENT FOR PRECONCENTRATION AND DETERMINATION OF TWO HORMONIC DRUGS (MEGESTROL ACETATE AND LEVONORGESTREL) BASED ON HOLLOW FIBER LIQUID-PHASE MICROEXTRACTION (HF-LPME) FOLLOWED BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) WITH UV-VIS DETECTION WAS APPLIED [2].THE EFFECT OF DIFFERENT EXTRACTION PARAMETERS SUCH AS FIBER LENGTH, EXTRACTION TIME, STIRRING RATE AND IONIC STRENGTH ON THE EXTRACTION EFFICIENCY WERE INVESTIGATED BY USING MODIFIED SIMPLEX AND CENTRAL COMPOSITE DESIGN METHOD AS A SCREENING METHOD AND OPTIMIZATION METHOD, RESPECTIVELY. THE EFFECT OF SUPPORTED LIQUID MEMBRANE POLARITY AND TYPE OF ACCEPTOR PHASE WERE OPTIMIZED WITH ONE VARIABLE AT A TIME. THE CALIBRATION CURVES WERE LINEAR IN THE RANGE OF 0.5 TO 125MG L-1. LOD FOR BOTH OF THE DRUGS WAS 0.5 PPB. THE APPLICABILITY OF THIS TECHNIQUE WAS EXAMINED BY ANALYZING DRUGS IN URINE, PLASMA AND WASTE WATER SAMPLES. THE RELATIVE RECOVERIES OF THE DRUGS WERE IN THE RANGE OF 90 TO 104 % THAT SHOWS THE CAPABILITY OF THE METHOD FOR EXTRACTION WITH SUITABLE CLEAN-UP OF THESE DRUGS FROM VARIOUS MATRICES.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

  • No record.
  • Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops