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Information Seminar Paper

Title

FABRICATION OF BISMUTH-CHITOSAN MODIFIED GLASSY CARBON ELECTRODE AND ITS APPLICATION IN MEASUREMENT OF TARTRAZINE SYNTHETIC DYE IN FOOD AND DRUG SAMPLES

Pages

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Abstract

 BISMUTH-FILM MODIFIED ELECTRODES (BIFE) HAVE BEEN USED AS A SUITABLE ALTERNATIVE FOR DROPPING MERCURY ELECTRODES (DME) SINCE 2000 [1, 2]. THESE ELECTRODES HAVE HIGH HYDROGEN OVER POTENTIAL AND LIMITED OXYGEN INTERFERENCES.SYNTHETIC DYES HAVE WIDE APPLICATIONS IN VARIOUS INDUSTRIES SUCH AS FOOD, DRUG AND COSMETICS.IN COMPARISON TO NATURAL COLORS, SYNTHETIC DYES HAVE ADVANTAGES SUCH AS: HIGH STABILITY WHEN THEY ARE EXPOSURE TO LIGHT, PH AND OXYGEN, BEING MONOTONOUS, LOW BIOLOGICAL CONTAMINATION AND LOW PRICE[3].TARTRAZINE (TRISODIUM (4E) -5-OXO-1- (4-SULFONATOPHENYL) -4- [(4-SULFONATOPHENYL) HYDRAZONO] -3-PYRAZOLECARBOXYLATE) IS A SYNTHETIC DYE AND IS USED IN FOOD AND DRUG SAMPLES. TARTRAZINE IS ALSO KNOWN AS E102 AND ACID YELLOW 5. ALLOWED USES AMOUNT OF THIS COLOR IS RECEIVING DAILY 7.5 MG PER KILOGRAM OF BODY. BUT OVERUSE OF IT IN PRODUCTS CAN CAUSE HARMFUL EFFECTS ON HUMAN HEALTH. SO MEASURING THIS COLOR THAT IS USED IN MANY PRODUCTSESPECIALLY FOOD AND DRUGS, IS THE MATTER OF THIS RESEARCH.IN THIS STUDY, GLASSY CARBON ELECTRODE IS MODIFIED WITH BISMUTH BY EX-SITU METHOD. MOREOVER, THIS ELECTRODE IS MODIFIED BY CHITOSAN. BECAUSE CHITOSAN IS KNOWN FOR FOLLOWING PROPERTIES: BIOLOGICAL COMPATIBLE WITH OTHER ELEMENTS AND NON-TOXICITY. ALSO IT DOESN’T CONTAMINATE ELECTRODE'S SURFACE. EFFECTS OF SOME PARAMETERS SUCH AS APPLIED POTENTIAL, BISMUTH CONCENTRATION, PH, ELECTROLYTE CONCENTRATION AND DEPOSITION TIME WERE STUDIED. THE OPTIMUM CONDITIONS ARE: ACETATE BUFFER SOLUTION 0.1M (PH=4.5) CONTAINING 0.18 MG/ML BI+3 IONS BY ELECTROLYSIS AT -0.75 V VS.SCE FOR 200S, WHILE STIRRING THE SOLUTION.ELECTROCHEMICAL BEHAVIOR OF TARTRAZINE WAS INVESTIGATED ON THE MODIFIED ELECTRODE.ALSO, TARTRAZINE WAS DETERMINED IN FOOD AND DRUG SAMPLES ON BISMUTH-CHITOSAN MODIFIED GLASSY CARBON ELECTRODE BY DIFFERENTIAL PULSE VOLTAMMETRY (DPV) TECHNIQUE.

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