Seminar Paper

Paper Information

video

sound

Persian Version

View:

1,122

Download:

Cites:

Information Seminar Paper

Title

EXTRACTION OF OLEUROPEIN FROM OLEA UROPEA USING MSPD METHOD AND MEASURED WITH HPLC

Pages

 Start Page | End Page

Keywords

Not Registered.

Abstract

 MATRIX SOLID-PHASE DISPERSION (MSPD) METHODOLOGY AS A QUICK AND EASY EXTRACTION METHOD HAS BEEN DEVELOPED TO EXTRACT OLEUROPEIN FROMOLEA EUROPAEA LEAVES. THIS METHOD HAS BEEN COMPARED WITH CONVENTIONAL MACERATION AND SOXHLET EXTRACTION (SE) METHODS [1]. VARIABLES AFFECTING MSPD, SUCH AS THE SORBENT MATERIAL, THE RATIO OF SAMPLE TO SORBENT MATERIAL, ELUTION SOLVENT, AND VOLUME OF THE ELUTION SOLVENT HAVE BEEN FULLY EVALUATED AND OPTIMIZED. OLEUROPEIN WAS SUCCESSFULLY EXTRACTED BY SILICA AS SORBENT WITH 350ML OF DICHLOROMETHANE- METHANOL (70: 30, V/V) AS ELUTION SOLVENT. THE CALIBRATION CURVE SHOWED GOOD LINEARITY (R 2=0.9985) AND PRECISION (RSD<4.5%) IN THE CONCENTRATION RANGE OF 0.5-100 MG/ML FOR OLEUROPEIN. THE LIMIT OF DETECTION (LOD) AND LIMIT OF QUANTICATION (LOQ) WERE 0.14 AND 0.5 MG/ML, RESPECTIVELY. THE RECOVERIES WERE IN THE RANGE OF 88.0-94.0% WITH RSD VALUES RANGING FROM 6.0 TO 9.8%. THE EXTRACTION YIELDS OBTAINED BY THE MSPD METHOD WERE COMPARABLE WITH THOSE OBTAINED BY THE MACERATION AND SE EXTRACTION METHODS. THE REPORTED MSPD EXTRACTION METHOD REVEALED TO BE ECO-FRIENDLY, SIMPLER AND FASTER THAN CONVENTIONAL METHODS USED TO QUANTIFY OLEUROPEIN IN OLEA EUROPAEALEAVES.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

  • No record.
  • Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops