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Title

GENOMIC COMPARISON OF MYCOBACTERIUM TUBERCLOSIS ISOLATED FROM PATIENT IN GOLESTAN PROVINCE (NORTH OF IRAN)

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Abstract

 TUBERCLOSIS (TB) CAUSED BY MYCOBACTERIUM TUBERCULOSIS WITH ALMOST 9 MILION NEW CASES EACH YEAR REMAINS OF THE MOST FEARED DISEASE ON THE PLANET. THE IDENTIFICATION OF SOURCES OF INFECTION, ROUTES OF TRANSMISSION ARE IMPORTANT PART OF AN ERADICATION SCHEME AND THESE CAN BE ACHIEVED BY DIFFERENTIATION OF MYCOBACTERIUM TUBERCULOSIS ISOLATES BY MOLECULAR TYPING METHOD SUCH AS RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP). GOLESTAN PROVINCE AFTER SISTAN AND BALOOCHESTAN HAS THE HIGHEST NUMBER OF TB PATIENT IN IRAN .IN THIS STUDY RFLP TEQNIQUE BYTWO ENZYMES PVU II AND ALU 1 AND PGRS PROBE WERE COMPARED OF GENOMIC PATTERN DIVERSITY AND BETTER IDENTIFY STRAIN. FIVE SPUTUM SPECIEMENT WERE COLLECTED FROM SMEAR POSITIVE PATIENT IN GOLESTAN PROVINCE. SAMPLES WERE PROCESSED AND CULTURE ON LOWENSTEIN JENSEN (LJ) SLANT FOR THREE WEEKS. DNA WAS EXTRACTED FROM TWO SAMPLE BASED ON VAN EMBDEN PROTOCOL. FIRST PCR ASSAY TARGETING 16S RRNA GENES WAS USED FOR IDENTIFICATION OF MYCOBACTERIUM SPP. WHOLE DNA RFLP WITH PVU II AND ALU 1 RESTRICTION ENZYME AND HYBRIDIZED WITH DIG LABELED PGRS PROBE. IN THIS STUDY, FROM 5 ISOLATES, 2 DIFFERENT GENOMIC PATTERNWERE OBTAINED AFTER DIGESTION WITH PVU II AND HYBRIDIZATION WITH PGRS, AND 3 DIFFERENT GENOMIC PATTERNS WERE OBTAINED AFTER DIGESTION WITH ALU 1AND HYBRIDIZATION WITH PGRS.

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