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Title

APPLICATION OF 16S RRNA ANALYSIS ON IDENTIFICATION OF LUMINESCENT BACTERIA FROM SHRIMP FARMS IN PERSIAN GULF

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Abstract

 BACKGROUND AND PURPOSE:MOLECULAR METHODS ARE MOST SUITABLE TOOLS FOR S THE MICROBIAL DIVERSITY AND IDENTIFICATION. IN PRESENT STUDY, WE USED THE CONVENTIONAL MOLECULAR METHODS TO IDENTIFY LUMINESCENCE BACTERIA ISOLATED FROM SHRIMP PONDS OF THE PERSIAN GULF.MATERIALS AND METHODS:THE WATER SAMPLES WERE TAKEN FROM TWO FARMS (9 POOLS) IN BUSHEHR SHRIMP. THEIR ISOLATION AND PURIFICATION WAS PERFORMED USING SPECIFIC AGAR MEDIA CULTURE (TCBS AND SWC). THEIR LUMINESCENCE WAS MEASURED BY BIO-LUMINOMETRY. THE EXTRACTED DNA WAS USED TO AMPLIFY THE 16S RRNA BY UNIVERSAL PRIMERS. THE PARTIAL 16S RRNA SEQUENCES OF THE ISOLATES WERE COMPARED WITH GENEBANK DATABASE.RESULTS:ACCORDING TO THE ANALYSIS OF 16S RRNA, THREE TYPES OF BIOLUMINESCENCE BACTERIA, WERE RELATED TO VIBRIO SP. ISOLATES OF 1A, 1B AND 2B SHOWED 99% SIMILARITY WITH VIBRIO ALGINOLYTICUS, VIBRIO OWENSII, VIBRIO COMMUNIS RESPECTIVELY.DISCUSSION:BLAST RESULTS SHOW THAT THREE SPECIES ARE BELONGED TO VIBRIO HARVEYI CLADE. ISOLETES OF 1B AND 2B ARE SIMILAR TOGETHER AND CLOSELY RELATED TO VIBRIO COMMUNIS. THE SEQUENCE OF 1A, WAS RELATED TO STRAIN ANCESTOR, VIBRIO HARVEYI.CONCLUSION:STRAINS OF VIBRIO HARVEYI AS A SPECIES IS CONSIDERED AS THE ANCESTRAL SPECIES THAT ARE SEPARATED AND DIFFERENCIATED IN THE EVOLUTION TIME BUT, IT SEEMS THAT THE EVOLUTION TIME IS VERY SHORT FOR THE NEWLY ISOLATES.

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